表面增强激光解吸/电离技术在筛查习惯性饮酒者中的诊断价值

Diagnostic values of surface-enhanced laser desorption/ionization technology for screening of habitual drinkers.

作者信息

Sogawa Kazuyuki, Itoga Sakae, Tomonaga Takeshi, Nomura Fumio

机构信息

Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.

出版信息

Alcohol Clin Exp Res. 2007 Jan;31(1 Suppl):S22-6. doi: 10.1111/j.1530-0277.2006.00282.x.

DOI:10.1111/j.1530-0277.2006.00282.x

PMID:17331162

Abstract

BACKGROUND

The currently available biological markers are limited in sensitivity and specificity for screening of excessive alcohol drinkers. We recently purified and identified 3 potential markers for alcoholism (5.9 and 7.8 kDa peptides and 28 kDa protein) by using surface-enhanced laser desorption/ionization (SELDI-TOF MS) technology. In the present study, we determined the diagnostic values of these novel markers in screening habitual drinkers with moderate alcohol consumption (hereafter habitual drinkers) in the general population as compared with 2 conventional markers: gamma-glutamyltransferase (GGT) and carbohydrate-deficient transferrin (CDT).

METHODS

Serum samples obtained from a total of 128 Japanese who sought regular medical checkup were analyzed by SELDI-TOF MS.

RESULTS

The relative intensities of the 5.9 and the 28 kDa peak of habitual drinkers were significantly different from those in nondrinkers even in GGT nonresponders. The sensitivity and specificity were the highest in 5.9 kDa. When 5.9, 28 kDa, and GGT were combined, 96.8% of the habitual drinkers were successfully screened with a specificity of 60.9%.

CONCLUSION

The results of this study clearly indicate that the 5.9 kDa peak is a promising novel biological marker for moderate alcohol consumption.

摘要

背景

目前可用的生物标志物在筛查过度饮酒者时，其敏感性和特异性有限。我们最近利用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF MS）技术纯化并鉴定出3种酒精中毒潜在标志物（5.9 kDa和7.8 kDa肽以及28 kDa蛋白）。在本研究中，我们测定了这些新型标志物相对于2种传统标志物：γ-谷氨酰转移酶（GGT）和缺糖转铁蛋白（CDT），在筛查普通人群中中度饮酒的习惯饮酒者（以下简称习惯饮酒者）时的诊断价值。