对乙酰氨基酚抑制小鼠脊髓切片中的一氧化氮合成。
Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.
作者信息
Godfrey Lisa, Bailey Ian, Toms Nick J, Clarke Geoffrey D, Kitchen Ian, Hourani Susanna M O
机构信息
Pharmacology Group, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
出版信息
Eur J Pharmacol. 2007 May 7;562(1-2):68-71. doi: 10.1016/j.ejphar.2007.01.075. Epub 2007 Feb 8.
Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.
对乙酰氨基酚是一种有效的镇痛药，但其作用机制尚不清楚。我们通过测量[(3)H]精氨酸向[(3)H]瓜氨酸的转化，研究了对乙酰氨基酚和镇痛佐剂咖啡因对体外培养的小鼠脊髓和小脑切片中一氧化氮合酶活性的影响。对乙酰氨基酚（100 microM）对小脑一氧化氮合酶活性没有影响，但在脊髓中，对乙酰氨基酚（100 microM）和咖啡因（30 microM）均减弱了谷氨酸（5 mM）诱导的[(3)H]瓜氨酸生成，两者联合使用则完全消除了这种生成。总之，对乙酰氨基酚抑制脊髓一氧化氮的合成，这可能与其镇痛作用有关。
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