Peterhaensel Christoph, Weier Dagmar, Lahaye Thomas
RWTH Aachen University, Biology I, Germany.
Methods Mol Biol. 2007;353:69-78. doi: 10.1385/1-59745-229-7:69.
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
当将非放射性检测方法与植物核酸结合使用时，会遇到几个特定的问题。在本章中，我们描述了从植物叶片中分离DNA和RNA的方法，以及通过PCR或用不同半抗原进行体外转录制备探针分子的方法。此外，还给出了探针杂交和检测的标准条件以及可能的改进方法。
