来自植物样本的非放射性Northern和Southern分析。
Nonradioactive northern and Southern analyses from plant samples.
作者信息
Peterhaensel Christoph, Weier Dagmar, Lahaye Thomas
机构信息
RWTH Aachen University, Biology I, Germany.
出版信息
Methods Mol Biol. 2007;353:69-78. doi: 10.1385/1-59745-229-7:69.
PMID:17332633
Abstract
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
摘要
当将非放射性检测方法与植物核酸结合使用时，会遇到几个特定的问题。在本章中，我们描述了从植物叶片中分离DNA和RNA的方法，以及通过PCR或用不同半抗原进行体外转录制备探针分子的方法。此外，还给出了探针杂交和检测的标准条件以及可能的改进方法。
相似文献
1
Nonradioactive northern and Southern analyses from plant samples.来自植物样本的非放射性Northern和Southern分析。
Methods Mol Biol. 2007;353:69-78. doi: 10.1385/1-59745-229-7:69.
2
Nonradioactive Northern blot analysis of plant RNA and the application of different haptens for reprobing.植物RNA的非放射性Northern印迹分析及不同半抗原用于再杂交的应用。
Anal Biochem. 1998 Nov 15;264(2):279-83. doi: 10.1006/abio.1998.2838.
3
Nonradioactive northern blotting.非放射性Northern印迹法。
Methods Mol Biol. 1998;86:77-86. doi: 10.1385/0-89603-494-1:77.
4
Multiple-sandwich, one-step hybridization of northern and Southern blots.
Biotechniques. 1995 Apr;18(4):585-6.
5
Isolation of plant mitochondrial RNA from green leaves.从绿叶中分离植物线粒体RNA。
Methods Mol Biol. 1998;86:33-8. doi: 10.1385/0-89603-494-1:33.
6
A reliable and efficient method for total rna isolation from various members of spurge family (Euphorbiaceae).一种从大戟科（Euphorbiaceae）各成员中分离总 RNA 的可靠且高效的方法。
Phytochem Anal. 2010 Sep-Oct;21(5):395-8. doi: 10.1002/pca.1205.
7
Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam.从甘薯（Ipomoea batatas (L.) Lam.）中快速可靠地提取DNA和RNA的方法
Biotechnol Lett. 2005 Dec;27(23-24):1841-5. doi: 10.1007/s10529-005-3891-2.
8
Extraction of plant RNA.植物RNA的提取
Methods Mol Biol. 2007;353:15-24. doi: 10.1385/1-59745-229-7:15.
9
"Sandwich" method for nonradioactive hybridization.
Biotechniques. 1995 May;18(5):800-2.
10
Nonradioactive Northern blotting with biotinylated and digoxigenin-labeled RNA probes.使用生物素化和地高辛标记的RNA探针进行非放射性Northern印迹分析。
Electrophoresis. 1998 Jun;19(8-9):1351-5. doi: 10.1002/elps.1150190825.
Zotero 插件