Sasaki Kengo, Haruta Shin, Ueno Yoshiyuki, Ishii Masaharu, Igarashi Yasuo
Department of Biotechnology, Graduate School of Agricultural and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Appl Microbiol Biotechnol. 2007 Jun;75(4):941-52. doi: 10.1007/s00253-007-0888-x. Epub 2007 Mar 3.
An anaerobic packed-bed reactor using carbon fiber textiles (CFT) as the supporting material was continuously operated using an artificial garbage slurry. 16S rRNA gene analysis showed that many bacteria in the biomass adhering to CFT were closely related to those observed from other anaerobic environments, although a wide variety of unidentified bacteria were also found. Dot blot hybridization results clarified that 16S rRNA levels of methanogens in the adhering biomass were higher than those in the effluent. Based on microscopic observation, the adhering biomass consisted of microorganisms, organic material, and void areas. Bacteria and Archaea detected by fluorescence in situ hybridization were distributed from the surface to the inner regions of the adhering biomass. Methanosarcina sp. tended to be more abundant in the inner part of the adhering biomass than at the surface. This is the first report to elucidate the structure of the microbial community on CFT in a packed-bed reactor.
使用碳纤维织物（CFT）作为支撑材料的厌氧填充床反应器，以人工垃圾浆为原料持续运行。16S rRNA基因分析表明，附着在CFT上的生物质中的许多细菌与在其他厌氧环境中观察到的细菌密切相关，尽管也发现了各种各样未鉴定的细菌。斑点杂交结果表明，附着生物质中产甲烷菌的16S rRNA水平高于流出物中的水平。基于显微镜观察，附着生物质由微生物、有机物质和空隙区域组成。通过荧光原位杂交检测到的细菌和古菌分布在附着生物质的表面到内部区域。甲烷八叠球菌属在附着生物质内部比在表面更丰富。这是第一份阐明填充床反应器中CFT上微生物群落结构的报告。
