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几种芽孢杆菌孢子的磁化率定量分析:对分离和检测的意义

Quantification of magnetic susceptibility in several strains of Bacillus spores: implications for separation and detection.

作者信息

Melnik Kristie, Sun Jianxin, Fleischman Aaron, Roy Shuvo, Zborowski Maciej, Chalmers Jeffrey J

机构信息

Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, Ohio 43210, USA.

出版信息

Biotechnol Bioeng. 2007 Sep 1;98(1):186-92. doi: 10.1002/bit.21400.

DOI:10.1002/bit.21400
PMID:17335063
Abstract

Three strains of Bacillus: Bacillus atrophaeus (formally Bacillus globigii), Bacillus thuringiensis, and Bacillus cereus were tested for their intrinsic magnetic susceptibility. All three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as Cell Tracking Velocimetry. Energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, Fe and Mn, in the spore form of the bacteria. It was demonstrated that this magnetic susceptibility is sufficient to separate and deposit these spores on glass slides in a magnetic deposition system. These results indicate the potential to separate spores with intrinsic magnetic susceptibility directly out of water or air samples.

摘要

对三种芽孢杆菌菌株

萎缩芽孢杆菌(原称球状芽孢杆菌)、苏云金芽孢杆菌和蜡样芽孢杆菌进行了固有磁化率测试。当这三种菌株形成芽孢时,使用一种称为细胞追踪测速仪的仪器显示出显著的磁化率。能量色散光谱也证实了在细菌芽孢形式中存在顺磁性元素铁和锰。结果表明,这种磁化率足以在磁沉积系统中将这些芽孢分离并沉积在载玻片上。这些结果表明,有可能直接从水或空气样本中分离出具有固有磁化率的芽孢。

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