Dean S, Tscherwonyi P J, Riley W J
Department of Biochemistry, Royal Perth Hospital, Western Australia.
Clin Chem. 1992 Jan;38(1):119-22.
A sensitive and precise electrothermal atomic absorption spectrophotometric method for determining bismuth concentration is described. Protein precipitation and the use of a palladium modifier reduce the problems of foaming and permit the use of a higher ashing temperature. The detection limit of the assay is 0.9 nmol/L. Total CVs (intra- and interassay) for serum ranged from 3.5% to 15.1% and for urine from 4.8% to 14.5% at concentrations of 60.0 and 6.0 nmol/L, respectively. Analytical recoveries of bismuth added to serum and urine were 102% and 103% over the same range. The method is robust and reproducible and can be accurately calibrated with aqueous standards.
本文描述了一种灵敏且精确的电热原子吸收分光光度法，用于测定铋浓度。蛋白质沉淀法以及使用钯改性剂可减少起泡问题，并允许使用更高的灰化温度。该测定方法的检测限为0.9 nmol/L。血清中总变异系数（批内和批间）在浓度为60.0 nmol/L时范围为3.5%至15.1%，尿液在浓度为6.0 nmol/L时范围为4.8%至14.5%。在相同范围内，添加到血清和尿液中的铋的分析回收率分别为102%和103%。该方法稳健且可重现，并且可以用水溶液标准品进行准确校准。
