拟南芥微管相关蛋白18通过破坏皮层微管的稳定性在细胞定向生长中发挥作用。
Arabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 functions in directional cell growth by destabilizing cortical microtubules.
作者信息
Wang Xia, Zhu Lei, Liu Baoquan, Wang Che, Jin Lifeng, Zhao Qian, Yuan Ming
机构信息
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
出版信息
Plant Cell. 2007 Mar;19(3):877-89. doi: 10.1105/tpc.106.048579. Epub 2007 Mar 2.
Abstract
Microtubule-associated proteins (MAPs) play important roles in the regulation of microtubule function in cells. We describe Arabidopsis thaliana MAP18, which binds to microtubules and inhibits tubulin polymerization in vitro and colocalizes along cortical microtubules as patches of dot-like structures. MAP18 is expressed mostly in the expanding cells. Cells overexpressing MAP18 in Arabidopsis exhibit various growth phenotypes with loss of polarity. Cortical microtubule arrays were significantly altered in cells either overexpressing MAP18 or where it had been downregulated by RNA interference (RNAi). The cortical microtubules were more sensitive to treatment with microtubule-disrupting drugs when MAP18 was overexpressed, but more resistant when MAP18 was eliminated in cells expressing MAP18 RNAi. Our study demonstrated that MAP18 may play a role in regulating directional cell growth and cortical microtubule organization by destabilizing microtubules.
摘要
微管相关蛋白（MAPs）在细胞微管功能的调控中发挥着重要作用。我们描述了拟南芥MAP18，它能与微管结合并在体外抑制微管蛋白聚合，且作为点状结构斑块沿皮层微管共定位。MAP18主要在正在扩展的细胞中表达。在拟南芥中过表达MAP18的细胞表现出各种生长表型且极性丧失。在过表达MAP18的细胞或通过RNA干扰（RNAi）使其下调的细胞中，皮层微管阵列发生了显著改变。当过表达MAP18时，皮层微管对微管破坏药物的处理更敏感，但在表达MAP18 RNAi的细胞中消除MAP18时则更具抗性。我们的研究表明，MAP18可能通过使微管不稳定在调控细胞定向生长和皮层微管组织中发挥作用。
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