牛肝铁氧化还原蛋白cDNA的分子克隆及核苷酸序列；肝铁氧化还原蛋白与肾上腺铁氧化还原蛋白的一级结构相同

Molecular cloning and nucleotide sequences of bovine hepato-ferredoxin cDNA; identical primary structures of hepato- and adreno-ferredoxins.

Matsuo Y, Tomita S, Tsuneoka Y, Furukawa A, Ichikawa Y

Department of Biochemistry, Kagawa Medical School, Japan.

Int J Biochem. 1992 Feb;24(2):289-95. doi: 10.1016/0020-711x(92)90260-8.

Abstract

The ferredoxin from bovine liver mitochondria, so-called hepatoredoxin, was purified and characterized as to its molecular weight, optical absorption spectrum and amino acid composition. 2. These properties were found to be very similar to those of adreno-ferredoxin. 3. To clarify the molecular basis of tissue specificity, the ferredoxin clones were obtained from a bovine liver library and the cDNA sequence of hepato-ferredoxin was determined. 4. The nucleotide sequence of hepato-ferredoxin clone was found to be identical to that of adreno-ferredoxin clone except for a single nucleotide in the 3' non-translated region. 5. Identical amino acid sequence of the two ferredoxins was confirmed by determining the partial amino acid sequence of the purified hepato-ferredoxin. 6. The results indicated that the organ specific activity of purified ferredoxin could not be explained by the different primary structure nor different RNA processing. 7. Other factors may be involved in the tissue specific properties of ferredoxins.

摘要

对来自牛肝线粒体的铁氧化还原蛋白（即所谓的肝铁氧化还原蛋白）进行了纯化，并对其分子量、光吸收光谱和氨基酸组成进行了表征。2. 发现这些特性与肾上腺铁氧化还原蛋白的特性非常相似。3. 为了阐明组织特异性的分子基础，从牛肝文库中获得了铁氧化还原蛋白克隆，并测定了肝铁氧化还原蛋白的cDNA序列。4. 发现肝铁氧化还原蛋白克隆的核苷酸序列与肾上腺铁氧化还原蛋白克隆的核苷酸序列相同，只是在3'非翻译区有一个单核苷酸不同。5. 通过测定纯化的肝铁氧化还原蛋白的部分氨基酸序列，证实了两种铁氧化还原蛋白的氨基酸序列相同。6. 结果表明，纯化的铁氧化还原蛋白的器官特异性活性不能用不同的一级结构或不同的RNA加工来解释。7. 其他因素可能与铁氧化还原蛋白的组织特异性特性有关。