Chin Keigi, Onishi Sachiko, Yuji Midori, Inamoto Tetsurou, Qi Wang-Mei, Yamamoto Kenkichi, Warita Katsuhiko, Yokoyama Toshifumi, Hoshi Nobuhiko, Kitagawa Hiroshi
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Japan.
J Vet Med Sci. 2007 Feb;69(2):193-9. doi: 10.1292/jvms.69.193.
Our previous study clarified that the apical regions of both the follicle-associated epithelium (FAE) of Peyer's patches and the intestinal villi are the only adhesion sites of indigenous bacteria in rat jejuno-ileum. To survey the ligands against bacterial lectins, sugar expression patterns on epithelial cells were lectin-histochemically investigated using 21 lectins in the jejuno-ileal Peyer's patches of rats. As a result, (D-glcNAc)(2-4), detected by Solanum tuberosum (STL) and by Lycopersicon esculentum (LEL), and beta-D-gal(1-3)-D-galNAc detected by Peanut agglutinin (PNA), were strongly expressed on the brush borders of the apical regions of the FAE and the intestinal villi. On the other hand, neither sugar was expressed on the brush borders of the basal regions of both FAE and intestinal villi. The positive intensities for the lectins correlated with the progression of epithelial apoptosis in the FAE and in the intestinal villi. Moreover, the double staining with lectin histochemical method and the in situ nick end-labeling method could simultaneously detect the strong expression of both sugars and nuclear DNA fragmentation in epithelial cells at the late apoptotic stage. Other sugar expression patterns in the intestinal villi were similar with those in the FAE. There were no lectins specific for M cells in the FAE. From these findings, the possible sugars of ligands against some indigenous bacterial lectins, expressing specially on the apoptotic epithelial cells, might be narrowed down in rat jejuno-ileum.
我们之前的研究表明，派尔集合淋巴结的滤泡相关上皮（FAE）和小肠绒毛的顶端区域是大鼠空肠回肠中固有细菌的唯一黏附位点。为了探寻针对细菌凝集素的配体，我们采用21种凝集素，通过凝集素组织化学方法研究了大鼠空肠回肠派尔集合淋巴结上皮细胞上的糖表达模式。结果显示，马铃薯凝集素（STL）和番茄凝集素（LEL）检测到的（D-葡萄糖胺）(2-4)，以及花生凝集素（PNA）检测到的β-D-半乳糖(1-3)-D-氨基半乳糖，在FAE和小肠绒毛顶端区域的刷状缘上强烈表达。另一方面，FAE和小肠绒毛基部区域的刷状缘均未表达这些糖类。凝集素的阳性强度与FAE和小肠绒毛上皮细胞凋亡的进程相关。此外，凝集素组织化学方法与原位缺口末端标记法的双重染色能够同时检测到凋亡后期上皮细胞中糖类的强烈表达和核DNA片段化。小肠绒毛中的其他糖表达模式与FAE中的相似。FAE中没有对M细胞特异的凝集素。基于这些发现，在大鼠空肠回肠中，针对某些固有细菌凝集素的配体可能的糖类，或许可以限定在特定凋亡上皮细胞上表达的糖类范围内。
