通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF质谱）和纳升液相色谱-电喷雾电离质谱（nano LC-ESI质谱）对cauxin的N-聚糖进行结构表征。
Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry.
作者信息
Suzuki Yusuke, Miyazaki Masao, Ito Emi, Suzuki Minoru, Yamashita Tetsuro, Taira Hideharu, Suzuki Akemi
机构信息
Sphingolipid Expression Laboratory, Supra-Biomolecular System Research Group, RIKEN Frontier Research System, Wako, Saitama, Japan.
出版信息
Biosci Biotechnol Biochem. 2007 Mar;71(3):811-6. doi: 10.1271/bbb.60599. Epub 2007 Mar 7.
Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Galbeta1-4GlcNAcbeta1-2Manalpha1-3(Galbeta1-4GlcNAcbeta1-2Manalpha1-6)(GlcNAcbeta1-4)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.
猫尿生长素是一种类似羧酸酯酶的糖蛋白，作为猫尿的主要成分排出。猫尿生长素含有四个假定的N-糖基化位点。我们使用纳升液相色谱（LC）-电喷雾电离（ESI）、基质辅助激光解吸/电离四极杆离子阱飞行时间质谱（MALDI-QIT-TOF MS）和MS/MS，以及配备十八烷基硅胶（ODS）柱的高效液相色谱（HPLC）对猫尿生长素的N-连接寡糖结构进行了表征。连接到（83）天冬酰胺上的猫尿生长素N-连接寡糖结构是一种平分复合型，即Galβ1-4GlcNAcβ1-2Manα1-3（Galβ1-4GlcNAcβ1-2Manα1-6）（GlcNAcβ1-4）Manβ1-4GlcNAcβ1-4（Fucα1-6）GlcNAc。
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