Yin Chih-Hsiu, Chen Wannhsin, Hsiao Chang-Chun, Kuo Chao-Ying, Chen Chao-Long, Wu Wen-Teng
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Biosci Biotechnol Biochem. 2007 Mar;71(3):728-34. doi: 10.1271/bbb.60568. Epub 2007 Mar 7.
Embryonic stem (ES) cells can differentiate into functional hepatic lineage cells, which can potentially be used in biomedicine. To obtain hepatic lineage cells from ES cells, embryoid bodies (EBs) must be formed. In this study, we developed an EB formation system using a spinner flask for mass production of EBs. ES cells were inoculated into the spinner flask, where they formed EBs within 4 d. The EBs were then transferred into an attached culture for hepatic differentiation. To verify the hepatic lineage cells, albumin secretion and hepatic-specific gene expression were examined. We found that EBs formed by either the spinner flask or hanging drops exhibited similar albumin secretion potential and hepatic-specific gene expression. We conclude that the spinner flask method can be used to produce mouse EBs that can be used to mass produce hepatic lineage cells for use in biomedicine.
胚胎干细胞（ES细胞）可分化为功能性肝系细胞，这些细胞有可能用于生物医学。为了从ES细胞中获得肝系细胞，必须形成胚状体（EBs）。在本研究中，我们开发了一种使用转瓶的EB形成系统，用于大规模生产EBs。将ES细胞接种到转瓶中，它们在4天内形成EBs。然后将EBs转移到贴壁培养中进行肝分化。为了验证肝系细胞，检测了白蛋白分泌和肝特异性基因表达。我们发现，通过转瓶或悬滴法形成的EBs表现出相似的白蛋白分泌潜力和肝特异性基因表达。我们得出结论，转瓶法可用于生产小鼠EBs，这些EBs可用于大规模生产用于生物医学的肝系细胞。
