[SD大鼠胰腺导管腺癌及癌旁胰腺组织中KiSS-1mRNA的表达]

[Expression of KiSS-1mRNA in pancreatic ductal adenocarcinoma and non-cancerous pancreatic tissues in SD rats].

作者信息

Liang Shan, Yang Zhu-Lin

机构信息

Clinical Institute of Medicine, Hunan Provincial People's Hospital, Changsha 410005, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Feb;32(1):109-13.

PMID:17344598

Abstract

OBJECTIVE

To establish a model of pancreatic cancer in Spragu-Dawely (SD) rats, and to examine the expression level of KiSS-1mRNA in pancreatic cancer and non-cancerous pancreatic tissues in SD rats.

METHODS

Dimethylbenzanthracene (DMBA) was directly implanted into the parenchyma of pancreas in SD rats (Group A), and DMBA combined with trichostatin (TSA) was implanted in the intervention group (Group B). The carcinogenesis of rats executed within 3 - 5 months in Group A and Group B were observed by HE staining and macrography. Meanwhile, the rats in the control (Group C) were executed in 5 months. The expression of KiSS-1mRNA was assayed by in situ hybridization.

RESULTS

(1) The incidence of pancreatic cancer in Group A within 3 - 5 months was 48.7% (18/37), including 17 cases of pancreatic ductal adenocarcinoma and 1 case of fibrosarcoma. The incidence of pancreatic cancer in Group B was 33.3% (12/36), including 11 pancreatic ductal adenocarcinoma and 1 fibrosarcoma. The maxial diameter of tumor mass in Group A was higher than that in Group B (P<0.05). (2) The positive rates of KiSS-1 mRNA in pancreatic cancer in Group A and Group B were significantly lower than those in non cancerous pancreatic tissues in Group A and Group B (P<0.01). The positive rates of KiSS-1mRNA in Group A or Group B with ductal adenocarcinoma were significantly lower than those in Group A or Group B without ductal adenocarcinoma (P<0.01). The middle or severely atypical ductal hyperplasia was observed in non-cancerous pancreatic tissues with the negative KiSS-1mRNA.

CONCLUSION

DMBA directly implanted into the parenchyma of pancreas can obtain an ideal pancreatic cancer model with high incidence in a short time. TSA may have an inhibitive effect on the carcinogenesis and the growth of pancreatic ductal adenocarcinoma in rats, and KiSS-1 may play an important role in inhibiting the invasion and metastasis of pancreatic cancer.

摘要

目的

建立斯普拉格 - 道利（SD）大鼠胰腺癌模型，并检测SD大鼠胰腺癌组织及癌旁胰腺组织中KiSS - 1mRNA的表达水平。

方法

将二甲基苯并蒽（DMBA）直接植入SD大鼠胰腺实质内（A组），干预组（B组）植入DMBA联合曲古抑菌素（TSA）。通过苏木精 - 伊红（HE）染色和大体观察，观察A组和B组3 - 5个月内处死大鼠的致癌情况。同时，对照组（C组）大鼠于5个月时处死。采用原位杂交法检测KiSS - 1mRNA的表达。

结果

（1）A组3 - 5个月内胰腺癌发生率为48.7%（18/37），其中胰腺导管腺癌17例，纤维肉瘤1例。B组胰腺癌发生率为33.3%（12/36），其中胰腺导管腺癌11例，纤维肉瘤1例。A组肿瘤块最大直径高于B组（P<0.05）。（2）A组和B组胰腺癌中KiSS - 1mRNA阳性率显著低于A组和B组癌旁胰腺组织（P<0.01）。A组或B组中伴有导管腺癌的KiSS - 1mRNA阳性率显著低于A组或B组中无导管腺癌者（P<0.01）。在KiSS - 1mRNA阴性的癌旁胰腺组织中观察到中度或重度非典型导管增生。