Danton Malcolm, Lim Chang Kee
MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
Biomed Chromatogr. 2007 May;21(5):534-45. doi: 10.1002/bmc.790.
Hydroxylated uroporphyrin I and urochlorin I derivatives formed by photochemical oxidation of uroporphyrinogen I were separated by high-performance liquid chromatography and fully characterized by electrospray ionization tandem mass spectrometry. The porphyrins and chlorins were identified by analysis of their product ion spectra with each hydroxylated derivative giving a characteristic collision-induced dissociation fragmentation pattern. The porphyrins and chlorins characterized were meso-hydroxyuroporphyrin I, alpha-hydroxypropionic acid uroporphyrin I, beta-hydroxypropionic acid uroporphyrin I, hydroxyacetic acid uroporphyrin I, trans-7-hydroxy-8-spirolactoneurochlorin I, cis-7-hydroxy-8-spirolactoneurochlorin I and trans- and cis-7,8-dihydroxyurochlorins I.
通过对尿卟啉原I进行光化学氧化形成的羟基化尿卟啉I和尿卟吩I衍生物，采用高效液相色谱法进行分离，并通过电喷雾电离串联质谱法进行全面表征。通过分析其产物离子光谱鉴定卟啉和二氢卟吩，每种羟基化衍生物都给出特征性的碰撞诱导解离碎裂模式。所表征的卟啉和二氢卟吩为中羟基尿卟啉I、α-羟基丙酸尿卟啉I、β-羟基丙酸尿卟啉I、羟基乙酸尿卟啉I、反式-7-羟基-8-螺内酯尿卟吩I、顺式-7-羟基-8-螺内酯尿卟吩I以及反式和顺式-7,8-二羟基尿卟吩I。
