Niedźwiadek Justyna, Mazur Elzbieta, Terlecki Piotr, Ziemba Barbara, Ligieza Jerzy, Wroński Jacek, Kozioł-Montewka Maria
Department of Microbiology, Medical University of Lublin, Poland.
Pol Merkur Lekarski. 2006 Nov;21(125):423-8.
of our study was the identification of microorganisms causing vascular graft infections and the evaluation of their antimicrobial susceptibility.
25 patients with infected vascular graft, took part in our research. In 19 patients late type of infection was recognized, in 6 the infection was qualified as early. Purulent discharge obtained from the fistula was inoculated on the bacteriological media. Antimicrobial susceptibility was assessed by disc-diffusion method.
Staphylococcus aureus and Pseudomonas aeruginosa proved to be the most frequently isolated microorganisms. Mixed infection, caused by two distinct bacteria, occurred in 5 patients; in all cases one species belonged to Gram-positive, and the second one to Gram-negative bacteria. In 50% of patients with early type infection different species of Gram-negative rods were present, in 37,5% of them S. aureus and S. epidermidis were isolated. In late type infection Gram-negative rods were isolated from 54,5% of patients and Gram-positive bacteria from 31,5% of patients. The most frequently isolated species appeared to be Pseudomonas aeruginosa. The isolated species of bacteria varied depending on the degree of infection (according to Shilagy and Samson).
A diversity of isolated species, the presence of mixed infections and resistance patterns typical for hospital flora among bacteria infecting vascular grafts cause that antibiotic therapy should always be based on the results of microbiological examination.
本研究的目的是鉴定引起血管移植物感染的微生物，并评估它们的抗菌药敏性。
25例血管移植物感染患者参与了我们的研究。其中19例为晚期感染，6例为早期感染。从瘘管获取的脓性分泌物接种于细菌培养基上。采用纸片扩散法评估抗菌药敏性。
金黄色葡萄球菌和铜绿假单胞菌是最常分离出的微生物。5例患者发生了由两种不同细菌引起的混合感染；在所有病例中，一种细菌属于革兰氏阳性菌，另一种属于革兰氏阴性菌。在50%的早期感染患者中存在不同种类的革兰氏阴性杆菌，37.5%的患者分离出金黄色葡萄球菌和表皮葡萄球菌。在晚期感染中，54.5%的患者分离出革兰氏阴性杆菌，31.5%的患者分离出革兰氏阳性菌。最常分离出的菌种似乎是铜绿假单胞菌。分离出的细菌种类因感染程度（根据希拉吉和萨姆森的标准）而异。
感染血管移植物的细菌种类多样、存在混合感染以及具有医院菌群典型的耐药模式，这使得抗生素治疗应始终基于微生物学检查结果。
