ławicki Sławomir, Czygier Małgorzata, Gacuta-Szumarska Ewa, Knapp Paweł, Szmitkowski Maciej
Department of Biochemical Diagnostics, Medical University of Białystok, Poland.
Pol Merkur Lekarski. 2006 Nov;21(125):465-8.
G-CSF and M-CSF are of the glycoproteins called colony-stimulating factors (CSFs). Some clinical investigations have shown an autologous production of G-CSF and M-CSF in various human cell lines in vitro and by tumors in vivo.
We have investigated the plasma levels and diagnostic utility of G-CSF M-CSF and commonly accepted tumor marker, such as CA 125 in ovarian cancer patients before surgery and healthy subjects.
We tested 30 patients with ovarian cancer and 30 healthy women (control group). G-CSF and M-CSF were determined using enzyme-linked immunosorbent assay (ELISA), CA 125 was measured by microparticle enzyme immunoassay (MEIA).
G-CSF, M-CSF and CA 125 plasma levels were significantly higher in ovarian cancer patients compared to the control group. The diagnostic sensitivity of M-CSF was higher than for G-CSF and CA 125 (57%, 50% i 53%, respectively). The diagnostic specificity was high and equal for all tested parameters (93%). Positive predictive value was higher for M-CSF and CA 125 (equal 89%). Negative predictive value was the highest for M-CSF in comparison to G-C.SF and CA 125 (68%, 65%, 66,7%, respectively).
Our study suggests that especially M-CSF can be clinically useful in diagnostic ovarian cancer, but further investigation and confirmation by a prospective study is necessary.
粒细胞集落刺激因子（G-CSF）和巨噬细胞集落刺激因子（M-CSF）属于被称为集落刺激因子（CSF）的糖蛋白。一些临床研究表明，在体外多种人类细胞系以及体内肿瘤中可出现G-CSF和M-CSF的自体产生。
我们研究了卵巢癌患者术前及健康受试者血浆中G-CSF、M-CSF以及常用肿瘤标志物（如CA 125）的水平及诊断效用。
我们检测了30例卵巢癌患者和30名健康女性（对照组）。采用酶联免疫吸附测定（ELISA）法测定G-CSF和M-CSF，采用微粒子酶免疫测定（MEIA）法测定CA 125。
与对照组相比，卵巢癌患者血浆中G-CSF、M-CSF和CA 125水平显著更高。M-CSF的诊断敏感性高于G-CSF和CA 125（分别为57%、50%和53%）。所有检测参数的诊断特异性均较高且相等（93%）。M-CSF和CA 125的阳性预测值更高（均为89%）。与G-CSF和CA 125相比，M-CSF的阴性预测值最高（分别为68%、65%、66.7%）。
我们的研究表明，尤其是M-CSF在卵巢癌诊断中可能具有临床应用价值，但有必要通过前瞻性研究进行进一步调查和确认。
