Torgonskaia M L, Firsova Iu E, Doronina N V, Trotsenko Iu A
Prikl Biokhim Mikrobiol. 2007 Jan-Feb;43(1):53-8.
A shortening of the lag phase in dichloromethane (DCM) consumption was observed in the methylobacteria Methylopila helvetica DM6 and Albibacter methylovorans DM10 after prior growth on methanol with the presence of 1.5% NaCI. Neither heat nor acid stress accelerated methylobacterium adaptation to DCM consumption. Sodium azide (1 mM) and potassium cyanide (1 mM) inhibited consumption of DCM by these degraders but not by transconjugants Methylobacterium extorquens AM1, expressing DCM dehalogenase but unable to grow on DCM. This indicates that the degrader strains possess energy-dependent systems of transport of DCM or chloride anions produced during DCM dehalogenation. Inducible proteins were found in the membrane fraction of A. methylovorans DM10 cells adapted to DCM and elevated NaCl concentration.
在1.5%氯化钠存在的情况下，甲基杆菌Methylopila helvetica DM6和Albibacter methylovorans DM10在甲醇上预先生长后，观察到二氯甲烷（DCM）消耗的延迟期缩短。热应激和酸应激均未加速甲基杆菌对DCM消耗的适应。叠氮化钠（1 mM）和氰化钾（1 mM）抑制了这些降解菌对DCM的消耗，但对表达DCM脱卤酶但不能在DCM上生长的转接合子Methylobacterium extorquens AM1没有抑制作用。这表明降解菌株拥有能量依赖的DCM或DCM脱卤过程中产生的氯离子阴离子转运系统。在适应DCM和升高的氯化钠浓度的Albibacter methylovorans DM10细胞的膜部分中发现了可诱导蛋白。
