Trufanov V A, Permiakova M D, Pshenichnikova T A, Ermakova M F, Davydov V A, Permiakov A V, Berezovskaia E V
Prikl Biokhim Mikrobiol. 2007 Jan-Feb;43(1):102-8.
The effects of intercultivar substitution of individual chromosome pairs (except for 1B, 6D, and 7A) in the wheat cultivars Saratovskaya 29 and Janetzkis Probat, differing in quality, on specific lipoxygenase activity, the grain yield per spike, and the main technological properties of flour and dough were studied. It was demonstrated that the substitution of individual chromosomes of the recipient cultivar Saratovskaya 29 with the homologous chromosomes of the donor cultivar Janetzkis Probat caused significant changes in lipoxygenase activity and several other quality characteristics. The correlations between the lipoxygenase activity and the parameters of physical dough properties were determined. Three molecular forms of lipoxygenase (Lpx-1, Lpx-2, and Lpx-3), differing in the value of surface charge and enzymatic activity, were detected by native PAGE.
研究了在品质不同的小麦品种萨拉托夫斯卡娅29号和扬采茨基斯·普罗巴特中，除1B、6D和7A染色体对外的单个染色体对的品种间替换，对特定脂氧合酶活性、每穗粒重以及面粉和面团主要工艺特性的影响。结果表明，用供体品种扬采茨基斯·普罗巴特的同源染色体替换受体品种萨拉托夫斯卡娅29号的单个染色体，会导致脂氧合酶活性和其他几个品质特性发生显著变化。确定了脂氧合酶活性与面团物理特性参数之间的相关性。通过非变性聚丙烯酰胺凝胶电泳检测到三种表面电荷值和酶活性不同的脂氧合酶分子形式（Lpx-1、Lpx-2和Lpx-3）。
