Kurosaka Nozomu, Bolte Andrea, Ali Marina, Manolios Nicholas
Department Orthopaedic Surgery, Fukuura Kanazawa-ku, Yokohama City University, Yokohama, Japan.
Protein Pept Lett. 2007;14(3):299-303. doi: 10.2174/092986607780090865.
A synthetic peptide termed core peptide (CP), which corresponds to a specific sequence of the TCR-alpha chain transmembrane domain, is known to inhibit IL-2 production in antigen stimulated T-cells. The molecular mechanism of the TCR inhibition is not known. This study examined the effects of CP on TCR subunit assembly and TCR cell surface expression in vitro. Co-transfection experiments between TCR-alpha and CD3-delta using COS-7 cells, and the interaction between TCR-alpha and the CD3 proteins in a T-cell line (2B4) were analysed after incubation with CP or its conjugates. Results indicate that CP co-precipitates with CD3-delta and CD3-epsilon in vitro, without any effect on TCR-alpha/CD3-delta dimerisation or TCR multisubunit assembly and cell surface expression.
一种称为核心肽（CP）的合成肽，它对应于TCR-α链跨膜结构域的特定序列，已知其可抑制抗原刺激的T细胞中IL-2的产生。TCR抑制的分子机制尚不清楚。本研究在体外检测了CP对TCR亚基组装和TCR细胞表面表达的影响。在用CP或其缀合物孵育后，分析了使用COS-7细胞进行的TCR-α和CD3-δ之间的共转染实验，以及T细胞系（2B4）中TCR-α与CD3蛋白之间的相互作用。结果表明，CP在体外与CD3-δ和CD3-ε共沉淀，对TCR-α/CD3-δ二聚化、TCR多亚基组装或细胞表面表达没有任何影响。
