Murtha Amy P, Feng Liping, Yonish Bryan, Leppert Phyllis C, Schomberg David W
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA.
Am J Obstet Gynecol. 2007 Mar;196(3):257.e1-5. doi: 10.1016/j.ajog.2007.01.007.
The purpose of this study was to determine whether progesterone exerts a protective effect in chorion and decidua cells when exposed to calcimycin.
Fetal membrane samples were collected from term elective repeat cesarean deliveries and chorion and decidua cells that are separated and cultured. Cells were pretreated with progesterone and exposed to calcimycin. Cell viability was determined, and percent cell viability was calculated.
Exposure to calcimycin resulted in a reduction of cell viability in both chorion and decidua cells in a dose-dependent fashion. In chorion and decidua cells, progesterone pretreatment followed by calcimycin increased cell viability compared with calcimycin treatment alone (chorion, 67%, vs controls, 24%; P < .001; decidua, 58%, vs controls, 35%; P < .001). The progesterone receptor antagonist, RTI 6413-49a, blocked the protective effect of progesterone in both chorion and decidua cells.
These preliminary results suggest that progesterone may provide a protective effect in fetal membrane cells and that this effect may be mediated through the progesterone receptor.
本研究旨在确定孕酮在暴露于钙离子载体A23187时是否对绒毛膜和蜕膜细胞发挥保护作用。
从足月择期再次剖宫产的胎膜样本中分离并培养绒毛膜和蜕膜细胞。细胞先用孕酮预处理，然后暴露于钙离子载体A23187。测定细胞活力，并计算细胞活力百分比。
暴露于钙离子载体A23187导致绒毛膜和蜕膜细胞的细胞活力以剂量依赖性方式降低。在绒毛膜和蜕膜细胞中，与单独使用钙离子载体A23187处理相比，先用孕酮预处理再用钙离子载体A23187处理可提高细胞活力（绒毛膜，67%，对照组为24%；P <.001；蜕膜，58%，对照组为35%；P <.001）。孕酮受体拮抗剂RTI 6413 - 49a可阻断孕酮在绒毛膜和蜕膜细胞中的保护作用。
这些初步结果表明，孕酮可能对胎膜细胞具有保护作用，且这种作用可能通过孕酮受体介导。
