Knight Brian S, Pennell Craig E, Adamson S Lee, Lye Stephen J
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
J Physiol. 2007 Jun 1;581(Pt 2):873-81. doi: 10.1113/jphysiol.2006.126573. Epub 2007 Mar 8.
The objective of this study was to characterize offspring responses to maternal dietary restriction (DR) in two phylogenetically distant strains of mice: A/J and C57BL/6J (B6). Pregnant mice were fed 100% or 70% of ad libitum between 6.5 and 17.5 days (d) gestation. Offspring were fed 100% ad libitum postweaning. All comparisons were made to strain and sex matched controls. Male DR-B6 offspring initially grew slower than controls; however, by 77 d and 182 d they were significantly heavier (P<0.05). Further, they had an increase percentage fat mass (+70%, P<0.01) by 182 d and were glucose intolerant at both 80 d (P<0.001) and 186 d (P<0.05). In contrast, weight, %Fat mass and glucose tolerance in DR-A/J males during postnatal life were not different from controls. Female DR-B6 mice showed catch-up growth during the first 77 d of life; however, their weight, %Fat mass and glucose tolerance were not different from controls at 80 d and 186 d. Although female DR-A/J were heavier than controls at 182 d (P<0.05), their %Fat mass and glucose tolerance were not different from controls at 182 d and 186 d. The observed strain and sex differences offer a unique opportunity to begin to define gene-environment interactions that contribute to developmental origins of health and disease.
A/J和C57BL/6J（B6）中，表征后代对母体饮食限制（DR）的反应。妊娠6.5至17.5天期间，给怀孕小鼠喂食自由采食量的100%或70%。断奶后给后代喂食自由采食量的100%。所有比较均与品系和性别匹配的对照组进行。雄性DR-B6后代最初生长比对照组慢；然而，到77天和182天时，它们明显更重（P<0.05）。此外，到182天时，它们的脂肪量百分比增加了70%（P<0.01），并且在80天（P<0.001）和186天（P<0.05）时均出现葡萄糖不耐受。相比之下，DR-A/J雄性后代出生后的体重、脂肪量百分比和葡萄糖耐量与对照组无差异。雌性DR-B6小鼠在出生后的前77天出现追赶生长；然而，在80天和186天时，它们的体重、脂肪量百分比和葡萄糖耐量与对照组无差异。尽管雌性DR-A/J在182天时比对照组重（P<0.05），但在182天和186天时，它们的脂肪量百分比和葡萄糖耐量与对照组无差异。观察到的品系和性别差异为开始定义导致健康和疾病发育起源的基因-环境相互作用提供了独特的机会。
