Polak Marta E, Borthwick Nicola J, Johnson Penny, Hungerford John L, Higgins Bernie, Di Palma Silvana, Jager Martine J, Cree Ian A
Translational Oncology Research Centre, Queen Alexandra Hospital, Southwick Hill, Portsmouth PO6 3LY, UK.
Br J Ophthalmol. 2007 Jul;91(7):971-6. doi: 10.1136/bjo.2006.110908. Epub 2007 Mar 8.
Uveal melanoma arises in an immune-privileged site and can itself add to the immunosuppressive environment. Previous studies on cutaneous melanoma have shown the presence of tolerogenic dendritic cells (DCs), which could play an important role in the progression of the tumour.
To examine the presence and functional status of DCs in a small series of uveal melanomas.
10 cases of uveal melanoma were examined for the expression of FXIIIa, CD68, human leucocyte antigen (HLA)-DR, CD40, CD83, transforming growth factor betaR1 and indolamine 2,3 dioxygenase by immunohistochemical analysis on sections embedded in paraffin wax.
CD68-positive macrophages were present in all of the tumours and were evenly distributed throughout. DCs expressing FXIIIa-positive were seen in 7 cases, and were often found concentrated in foci within the tumour mass. These cells were dendritic and expressed high levels of HLA-DR. The DCs did not express the maturation markers CD83 or CD40. In one case, concentration of DCs around the area of tumour necrosis was observed, and some of these cells expressed CD83.
Numerous tolerising antigen-presenting cells may play a role in melanoma-related immunosuppression in the eye, although activation of DCs may be associated with tumour necrosis.
葡萄膜黑色素瘤发生于免疫赦免部位，其本身可加剧免疫抑制环境。既往对皮肤黑色素瘤的研究显示存在耐受性树突状细胞（DCs），其可能在肿瘤进展中起重要作用。
检测一小系列葡萄膜黑色素瘤中DCs的存在情况及其功能状态。
采用免疫组织化学分析法，对10例葡萄膜黑色素瘤石蜡包埋切片检测FXIIIa、CD68、人类白细胞抗原（HLA）-DR、CD40、CD83、转化生长因子βR1和吲哚胺2,3-双加氧酶的表达。
所有肿瘤中均存在CD68阳性巨噬细胞，且均匀分布于肿瘤各处。7例可见表达FXIIIa阳性的DCs，常集中于肿瘤块内的病灶处。这些细胞呈树突状，且高表达HLA-DR。DCs不表达成熟标志物CD83或CD40。1例观察到肿瘤坏死区域周围有DCs聚集，其中部分细胞表达CD83。
众多耐受性抗原呈递细胞可能在眼部黑色素瘤相关免疫抑制中起作用，尽管DCs的激活可能与肿瘤坏死有关。
