Zhao Li Qing, Thiennu H Vu
Department of Biochemistry and Molecular Biology, Life Science College, nankai University, Tianjing.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2006 Dec;39(6):553-62.
Alveoli are the key functional units of the lungs where gas exchange takes place, yet little is known about the mechanisms of their formation. To identify the genes that regulate alveolar morphogenesis, we used suppression subtractive hybridization to isolate genes that differentially expressed during the formation of saccules and the remodeling of saccules into alveoli in the mouse. After differential screening, we got 118 candidate genes from two libraries. These genes associate with many aspects of life including proliferation, differentiation, regulation and so on. For example: Transient receptor protein 4(TRPC4) may associate with formation or remodeling of pulmonary vessels by increasing permeability of endothelia cells. Lectin, (galactose bindingl, Lgalsl) may play a significant role in stimulating smooth muscle growth in developing alveolar wall vessels and the development of pulmonary capillaries. Interesting, a putative neuronalprotein, P311 was found highly expressed in both the first and the secondary septation stages. Taking together SSH is a powerful method to identify differential expressing genes.
肺泡是肺部进行气体交换的关键功能单位，然而人们对其形成机制知之甚少。为了鉴定调控肺泡形态发生的基因，我们利用抑制性消减杂交技术分离在小鼠囊泡形成以及囊泡重塑为肺泡过程中差异表达的基因。经过差异筛选，我们从两个文库中获得了118个候选基因。这些基因与生命的许多方面相关，包括增殖、分化、调控等。例如：瞬时受体蛋白4（TRPC4）可能通过增加内皮细胞的通透性与肺血管的形成或重塑相关。凝集素（半乳糖结合，Lgals1）可能在刺激发育中的肺泡壁血管平滑肌生长和肺毛细血管发育中起重要作用。有趣的是，一种假定的神经元蛋白P311在初级和次级分隔阶段均高表达。综上所述，抑制性消减杂交技术是鉴定差异表达基因的有力方法。
