Nakano Takuo, Ikawa Noriaki, Ozimek Lech
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
J Agric Food Chem. 2007 Apr 4;55(7):2714-26. doi: 10.1021/jf062987f. Epub 2007 Mar 10.
A 64 amino acid residue sialylated phosphorylated glycomacropeptide (GMP) from bovine sweet whey can be detected as a Coomassie blue-staining peptide by electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels. There is, however, limited information available concerning detection of GMP as a sialylated phosphorylated compound. Samples of GMP were electrophoresed on SDS-polyacrylamide gels or cellulose acetate strips (CAS). Immediately following electrophoresis, fractions obtained by cutting gels or strips were subjected to sialic acid determination by the thiobarbituric acid reaction and phosphorus determination by the malachite green dye reaction. Both determinations were found to be sensitive enough to detect approximately 20 and 40 microg of GMP in CAS and SDS gels, respectively. Further studies demonstrated that sialylated phosphorylated GMP can be detected on either SDS gels or CAS loaded with whey products or whey-added margarine residues.
通过在十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶上进行电泳，来自牛乳清的一种含64个氨基酸残基的唾液酸化磷酸化糖巨肽（GMP）可作为考马斯亮蓝染色肽被检测到。然而，关于将GMP作为唾液酸化磷酸化化合物进行检测的可用信息有限。将GMP样品在SDS-聚丙烯酰胺凝胶或醋酸纤维素条（CAS）上进行电泳。电泳后立即对通过切割凝胶或条带获得的组分进行硫代巴比妥酸反应测定唾液酸以及孔雀石绿染料反应测定磷。发现这两种测定方法都足够灵敏，分别可在CAS和SDS凝胶中检测到约20微克和40微克的GMP。进一步的研究表明，唾液酸化磷酸化的GMP可在加载有乳清产品或添加乳清的人造黄油残渣的SDS凝胶或CAS上被检测到。
