猪肝二氢嘧啶脱氢酶催化NADPH氧化的立体化学
Stereochemistry of NADPH oxidation by dihydropyrimidine dehydrogenase from pig liver.
作者信息
Podschun B
机构信息
Theodor-Boveri-Institut fuer Biowissenschaften (Biozentrum), Universitaet Wuerzburg, Germany.
出版信息
Biochem Biophys Res Commun. 1992 Jan 31;182(2):609-16. doi: 10.1016/0006-291x(92)91776-m.
Dihydropyrimidine dehydrogenase reduces uracil to 5,6-dihydrouracil in a strictly NADPH-dependent reaction. Either by analysing the 1H-NMR spectra of the NADP+ products formed or by determination of the kinetic isotope effects of stereospecifically deuterated coenzymes dihydropyrimidine dehydrogenase was found to abstract specifically the pro-S hydrogen of NADPH, making it a member of the B-side stereospecific class of dehydrogenases.
二氢嘧啶脱氢酶在严格依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的反应中将尿嘧啶还原为5,6 - 二氢尿嘧啶。通过分析所形成的烟酰胺腺嘌呤二核苷酸磷酸（NADP⁺）产物的¹H - 核磁共振（¹H - NMR）谱，或者通过测定立体特异性氘代辅酶的动力学同位素效应，发现二氢嘧啶脱氢酶特异性地提取NADPH的前手性S氢，使其成为B面立体特异性脱氢酶类的一员。
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