Robert R, Chapelain B, Jean T, Néliat G
CEREP, Celle l'Evescault, France.
Biochem Biophys Res Commun. 1992 Jan 31;182(2):733-9. doi: 10.1016/0006-291x(92)91793-p.
Incubation of rabbit aortic rings with interleukin-1 (100 U/ml) in vitro led to a depressed contractile response to norepinephrine, whether the endothelium was present or not. In both cases norepinephrine-induced contraction was restored in the presence of NG-methyl-L-arginine (300 microM), an inhibitor of nitric oxide synthesis. In interleukin-1-treated rings precontracted with norepinephrine (1 microM), the relaxing response to acetylcholine was totally suppressed independently on the presence of endothelium. High concentrations of acetylcholine (greater than 1 microM) induced a slight contraction which was of lower amplitude than that obtained in control endothelium-denuded rings and was increased in the presence of NG-methyl-L-arginine. These results show that interleukin-1 (i) affects not only vascular contraction but also relaxation and (ii) involves both endothelial and non-endothelial factors. These observations suggest an impairment of the whole vascular reactivity during septic shock.
体外将兔主动脉环与白细胞介素 -1（100 U/ml）孵育，无论有无内皮，均导致对去甲肾上腺素的收缩反应减弱。在这两种情况下，一氧化氮合成抑制剂NG - 甲基 -L - 精氨酸（300 μM）存在时，去甲肾上腺素诱导的收缩得以恢复。在预先用去甲肾上腺素（1 μM）预收缩的白细胞介素 -1处理的血管环中，对乙酰胆碱的舒张反应无论有无内皮均被完全抑制。高浓度乙酰胆碱（大于1 μM）诱导轻微收缩，其幅度低于对照去内皮环，且在NG - 甲基 -L - 精氨酸存在时增强。这些结果表明，白细胞介素 -1（i）不仅影响血管收缩，还影响舒张，（ii）涉及内皮和非内皮因素。这些观察结果提示脓毒性休克期间整个血管反应性受损。
