Li Yunmin, Vilain Eric, Conte Felix, Rajpert-De Meyts Ewa, Lau Yun-Fai Chris
Department of Medicine, VA Medical Center, University of California, San Francisco, CA 94121, USA.
Urol Oncol. 2007 Mar-Apr;25(2):141-6. doi: 10.1016/j.urolonc.2006.08.002.
The testis-specific protein Y-encoded gene (TSPY) is a tandem repeat gene located at the critical region for the gonadoblastoma locus on Y chromosome that predisposes the dysgenetic gonads of intersex individuals to oncogenesis. The expression and molecular properties of TSPY suggest that it is the putative gene for the gonadoblastoma locus on Y chromosome. In this study, we examined the expression of TSPY and other germ cell tumor markers in 4 cases of gonadoblastoma using immunostaining techniques. Our results showed that TSPY expression was closely associated with initiation and various stages of gonadoblastoma development. TSPY protein localized with established germ cell tumor markers, such as the placental alkaline phosphatase, c-KIT, and OCT3/4, in the same tumor cells of both gonadoblastoma and adjacent carcinoma in situ, the precursor for germ cell tumors. These findings support the candidacy of TSPY as the gene for the gonadoblastoma locus on Y chromosome and suggest that TSPY could be a significant marker for these types of germ cell tumors.
睾丸特异性蛋白Y编码基因（TSPY）是一种串联重复基因，位于Y染色体上性腺母细胞瘤位点的关键区域，该区域使两性畸形个体发育异常的性腺易于发生肿瘤。TSPY的表达和分子特性表明它是Y染色体上性腺母细胞瘤位点的推定基因。在本研究中，我们采用免疫染色技术检测了4例性腺母细胞瘤中TSPY及其他生殖细胞肿瘤标志物的表达。我们的结果表明，TSPY表达与性腺母细胞瘤发生及发展的各个阶段密切相关。在性腺母细胞瘤及相邻原位癌（生殖细胞肿瘤的前体）的相同肿瘤细胞中，TSPY蛋白与已确定的生殖细胞肿瘤标志物，如胎盘碱性磷酸酶、c-KIT和OCT3/4定位在一起。这些发现支持TSPY作为Y染色体上性腺母细胞瘤位点基因的候选资格，并表明TSPY可能是这些类型生殖细胞肿瘤的重要标志物。
