Hunt J S, Morales P J, Pace J L, Fazleabas A T, Langat D K
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Mail Stop 3038, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA.
Placenta. 2007 Apr;28 Suppl A(Suppl A):S57-63. doi: 10.1016/j.placenta.2007.01.004. Epub 2007 Mar 9.
HLA-G is an HLA class Ib gene that is highly expressed in human trophoblast cells. The single HLA-G mRNA is alternatively spliced to generate at least seven transcripts, three of which encode soluble isoforms. Many studies have shown that high levels of soluble antigens are associated with successful implantation and graft acceptance. To study expression, regulation and functions of two of the soluble isoforms, HLA-G5 and HLA-G6, we generated recombinant proteins in eukaryotic cells and developed monoclonal antibodies specific for each of the two proteins. In addition, we investigated the olive baboon Paan-AG gene as a potential functional correlate of HLA-G. Here, we present summaries of the studies that have been conducted in our laboratory using these tools and discuss the results within the context of the research on this topic that is ongoing in ours and other laboratories worldwide. Collectively, the data indicate that soluble HLA-G is a critical contributor to immune privilege in pregnancy and imply that this placenta-derived substance may impact other pathways leading to successful reproduction.
HLA - G是一种HLA Ib类基因，在人类滋养层细胞中高度表达。单一的HLA - G信使核糖核酸（mRNA）经过可变剪接产生至少七种转录本，其中三种编码可溶性异构体。许多研究表明，高水平的可溶性抗原与成功着床和移植物接受相关。为了研究两种可溶性异构体HLA - G5和HLA - G6的表达、调控及功能，我们在真核细胞中生成了重组蛋白，并开发了针对这两种蛋白各自的单克隆抗体。此外，我们研究了橄榄狒狒的Paan - AG基因，将其作为HLA - G的潜在功能相关物。在此，我们展示了在我们实验室使用这些工具所开展研究的总结内容，并在我们实验室及全球其他实验室正在进行的关于该主题的研究背景下讨论结果。总体而言，数据表明可溶性HLA - G是孕期免疫特惠的关键促成因素，并暗示这种源自胎盘的物质可能会影响导致成功繁殖的其他途径。
