Kouitcheu Mabeku Laure B, Penlap Beng V, Kouam J, Essame Oyono, Etoa F X
Laboratory of Microbiology, Department of Biochemistry, University of Yaoundé I, Cameroon.
J Ethnopharmacol. 2007 May 22;111(3):598-606. doi: 10.1016/j.jep.2007.01.010. Epub 2007 Jan 14.
The toxicity profile of the ethyl acetate (EA) extract of the stem bark of Cylicodiscus gabunensis (CG) was studied in Wistar rats. The rats were administered graded doses (0.75, 1.5, 3 and 6 g/kg p.o.) of the extract daily for 6 weeks and the effects on clinical signs, body weight, food and water consumption, organ weight, haematology, histology as well as serum, hepatic and renal biochemical parameters were measured. Body weight of dosed and control rats increase throughout the duration of treatment but food and water consumption were not significantly affected. The relative weights of the liver, lungs, heart and kidneys remained normal whereas a significant change was observed in that of the spleen. The hematocrit level was increased in treated animal. Our data demonstrates a significant increase in serum concentrations of aspartate amino-transferase, alanine amino-transferase, total cholesterol and glucose with high-dose of CG treatment tested (3-6 g/kg). CG also caused a significant reduction in hepatic malondialdehyde concentration. Renal urea and creatinine levels were reduced significantly in test groups. Histological findings reveal a characteristic progression treatment-related effect on liver, kidneys and lungs. The acute toxicity LD50 was estimated at 14.5 and 11 g/kg body weight (b.w.) for male and female respectively, but dose-related mortality of 30 and 50% were observed during the sub-acute toxicity. These findings have once more highlighted the limitations of the acute toxicity LD50 testing and suggest that CG may exert varied toxicological effects when administered orally in rats.
对加蓬轮盘木（CG）茎皮的乙酸乙酯（EA）提取物的毒性特征在Wistar大鼠中进行了研究。大鼠每天口服给予分级剂量（0.75、1.5、3和6 g/kg）的提取物，持续6周，并测量其对临床体征、体重、食物和水消耗、器官重量、血液学、组织学以及血清、肝脏和肾脏生化参数的影响。给药大鼠和对照大鼠的体重在整个治疗期间均增加，但食物和水的消耗未受到显著影响。肝脏、肺、心脏和肾脏的相对重量保持正常，而脾脏的相对重量则出现了显著变化。治疗动物的血细胞比容水平升高。我们的数据表明，在测试的高剂量CG治疗（3 - 6 g/kg）下，天冬氨酸氨基转移酶、丙氨酸氨基转移酶、总胆固醇和葡萄糖的血清浓度显著增加。CG还导致肝脏丙二醛浓度显著降低。测试组的肾脏尿素和肌酐水平显著降低。组织学结果揭示了对肝脏、肾脏和肺的与治疗相关的特征性进展性影响。急性毒性LD50估计雄性和雌性分别为14.5和11 g/kg体重（b.w.），但在亚急性毒性期间观察到30%和50%的剂量相关死亡率。这些发现再次突出了急性毒性LD50测试的局限性，并表明CG在大鼠口服给药时可能产生不同的毒理学效应。
