Rajesh Arora, Sagar R, Singh S, Kumar R, Sharma A K, Prasad J, Singh S, Gupta M, Sharma R K, Puri S C, Krishna B, Siddiqui M S, Lahiri S S, Tripathi R P, Qazi G N
Medicinal and Aromatic Plants Laboratory, Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Road, Delhi-110 054, India.
Integr Cancer Ther. 2007 Mar;6(1):54-65. doi: 10.1177/1534735406298303.
The radioprotective effect of a hydroalcoholic extracted material (REC-2000) from the rhizome of Podophyllum hexandrum was studied in mice exposed to lethal gamma radiation (10 Gy). The extract (REC-2000) was found to restore the hemoglobin content (14.73 +/- 0.33) and total leukocyte count (TLC) (4166.66 +/- 0.02) in lethally (10 Gy) gamma-irradiated mice on the 15th day in comparison to the radiation control mice. The hemoglobin content of the drug + radiation group was observed to be significantly (21.25%) higher than the radiation control group on the 10th day. Similarly, the TLC was significantly increased (83.33 times) in the drug + radiation group as compared to a radiation (10 Gy) only group on the 10th day. Enhanced expression of heme-oxygenase-1 and Bcl-2 protein observed by Western blotting further supports the observation of hemopoietic recovery in irradiated mice. These findings indicate that the bioactive constituents present in REC-2000 exert the radioprotective effect by modulating the hemopoietic system.
研究了从喜马拉雅鬼臼根茎中提取的水醇提取物（REC - 2000）对接受致死剂量γ射线照射（10 Gy）小鼠的辐射防护作用。与辐射对照组小鼠相比，发现提取物（REC - 2000）可使接受致死剂量（10 Gy）γ射线照射的小鼠在第15天时恢复血红蛋白含量（14.73±0.33）和总白细胞计数（TLC）（4166.66±0.02）。在第10天时，观察到药物 + 辐射组的血红蛋白含量比辐射对照组显著高（21.25%）。同样，在第10天时，与仅接受10 Gy辐射的组相比，药物 + 辐射组的TLC显著增加（83.33倍）。蛋白质印迹法观察到的血红素加氧酶 - 1和Bcl - 2蛋白表达增强进一步支持了受照射小鼠造血恢复的观察结果。这些发现表明，REC - 2000中存在的生物活性成分通过调节造血系统发挥辐射防护作用。
