Eissenberg Joel C, Lee Min Gyu, Schneider Jessica, Ilvarsonn Anne, Shiekhattar Ramin, Shilatifard Ali
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, USA.
Nat Struct Mol Biol. 2007 Apr;14(4):344-6. doi: 10.1038/nsmb1217. Epub 2007 Mar 11.
Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.
组蛋白H3赖氨酸4（H3K4）由酵母Set1-COMPASS及其哺乳动物同源物MLL复合物进行甲基化修饰。人类JARID1d能够使三甲基化的H3K4（H3K4me3）发生去甲基化。我们鉴定出果蝇的小成虫盘（Lid）是JARID1d的同源物。我们报告称，使用RNA干扰技术敲低Lid会导致全基因组范围内H3K4me3水平特异性升高，而不影响其他H3甲基化模式，并且会导致染色体解旋酶蛋白Chd1的分布发生改变。
