建立具有不同特征的人类癌细胞克隆：一种筛选化学预防剂的模型。
Establishment of human cancer cell clones with different characteristics: a model for screening chemopreventive agents.
作者信息
Ware Jeffrey H, Zhou Zhaozong, Guan Jun, Kennedy Ann R, Kopelovich Levy
机构信息
Department of Radiation Oncology, Division of Oncology Research, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104-6072, USA.
出版信息
Anticancer Res. 2007 Jan-Feb;27(1A):1-16.
BACKGROUND
The present study was undertaken in order to establish phenotypically different cell clones from 10 parental lines of human breast (MCF-7 and T-47D), prostate (PC-3 and DU145), lung (A549 and A427), colon (HCT-116 and HT-29) and bladder (TCCSUP and T24) cancer cells.
MATERIALS AND METHODS
Sublines were established from each of the parental lines by the limiting dilution method. The derived clones were characterized in terms of plating efficiency, cell proliferation rate, saturation density and colony formation efficiency in soft agar.
RESULTS
Phenotypically different cell clones were derived from each parental human cancer cell line, with many clones having more 'normal' characteristics than the parental line from which they were derived.
CONCLUSION
Phenotypically normal clones obtained through clonal selection from human cancer cell populations are expected to be a useful tool for the screening of cancer chemopreventive agents and the study of tumor progression.
背景
本研究旨在从人乳腺癌（MCF - 7和T - 47D）、前列腺癌（PC - 3和DU145）、肺癌（A549和A427）、结肠癌（HCT - 116和HT - 29）及膀胱癌（TCCSUP和T24）的10个亲代细胞系中建立表型不同的细胞克隆。
材料与方法
通过有限稀释法从每个亲代细胞系建立亚系。对所得克隆在接种效率、细胞增殖速率、饱和密度及软琼脂中的集落形成效率方面进行表征。
结果
从每个人类癌细胞亲代系中都获得了表型不同的细胞克隆，许多克隆具有比其来源的亲代系更多的“正常”特征。
结论
通过从人类癌细胞群体中进行克隆选择获得的表型正常的克隆有望成为筛选癌症化学预防剂及研究肿瘤进展的有用工具。
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