Ware Jeffrey H, Zhou Zhaozong, Kopelovich Levy, Kennedy Ann R
Department of Radiation Oncology, Division of Oncology Research, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104-6072, USA.
Anticancer Res. 2006 Nov-Dec;26(6B):4177-83.
The successful use of clonal selection through fluctuation analysis of human cancer cells as a means for studying tumor progression has been previously reported.
Three clones derived from a parental population of human prostate cancer (LNCaP) cells were selected based on proliferation, hormone sensitivity and anchorage-independent growth. The effects of five potential cancer preventive agents were evaluated using cell proliferation, anchorage-independent growth and apoptosis as end-points.
Clone 21 cells, which represent a presumptive normal phenotype, were generally more sensitive than Clone 17 and Clone 6 cells, which represent a more malignant phenotype, to fluasterone, 7beta-HF, L-selenomethionine and troglitazone in assays for proliferation and/or apoptosis.
The results confirm the efficacy of the above agents as cancer chemopreventive agents and support our contention that clonal selection of established human cancer cells provides a model to study the efficacy of chemopreventive agents.
先前已有报道称，通过对人类癌细胞进行波动分析成功运用克隆选择作为研究肿瘤进展的一种手段。
基于增殖、激素敏感性和不依赖贴壁生长，从人前列腺癌（LNCaP）细胞的亲代群体中选择了三个克隆。以细胞增殖、不依赖贴壁生长和凋亡作为终点，评估了五种潜在癌症预防剂的效果。
代表假定正常表型的克隆21细胞，在增殖和/或凋亡检测中，通常比代表更恶性表型的克隆17细胞和克隆6细胞对氟他胺、7β - HF、L - 硒代蛋氨酸和曲格列酮更敏感。
结果证实了上述试剂作为癌症化学预防剂的有效性，并支持我们的观点，即对已建立的人类癌细胞进行克隆选择提供了一个研究化学预防剂疗效的模型。
