König Frank, Krekeler Gisbert, Hönig Johannes F, Cordon-Cardo Carlos, Fischer Gösta, Korabiowska Monika
Multidisciplinary Research Center for Oral Diseases, Donau University, Krems, Austria.
Anticancer Res. 2007 Jan-Feb;27(1A):283-8.
Overexpression of p16 has been demonstrated to be strongly related to the presence of HPV16, 18. Squamous cell carcinoma of the head and neck has been shown to be associated with human papillomavirus (HPV) infection. The main aim of this study was to investigate the relationship between HPV presence and p16 expression in a representative collection of 60 head and neck carcinomas by tissue microarrays. The presence of HPV (HPV6, 11-low risk, HPV16, 18-high risk) was detected by applying in situ hybridisation. P-16 protein expression was detected immunohistochemically. HPV6, 11 positivity was observed in 10 out of 60 carcinomas. HPV16, 18 presence was found in 30 out of 60 tumours. P-16 expression was detected in 35 out of 60 tumours. A statistically significant relationship was demonstrated between HPV16, 18 presence and increased expression of p16. Also the HPV6, 11 presence was significantly correlated with p16 immunoreactivity. Additionally, this study demonstrates that it is possible to analyse p16 expression and HPV presence by tissue microarrays.
p16的过表达已被证明与HPV16、18的存在密切相关。头颈部鳞状细胞癌已被证明与人乳头瘤病毒（HPV）感染有关。本研究的主要目的是通过组织微阵列，在一组具有代表性的60例头颈部癌中研究HPV的存在与p16表达之间的关系。通过原位杂交检测HPV（HPV6、11-低风险型，HPV16、18-高风险型）的存在。通过免疫组织化学检测p-16蛋白的表达。在60例癌组织中有10例检测到HPV6、11阳性。在60例肿瘤中有30例发现HPV16、18存在。在60例肿瘤中有35例检测到p-16表达。HPV16、18的存在与p16表达增加之间存在统计学上的显著关系。HPV6、11的存在也与p16免疫反应性显著相关。此外，本研究表明通过组织微阵列分析p16表达和HPV的存在是可行的。
