[人乳头瘤病毒感染及p16蛋白在喉乳头状瘤和喉癌中的表达]

[Human papilloma virus infection and expression of p16 protein in laryngeal papilloma and laryngeal carcinoma].

作者信息

Li J, Yang J, Yi Z, Lin Y, Zhou A

机构信息

Department of Otorhinolaryngology, Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.

出版信息

Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Feb;36(1):51-4.

PMID:12761910

Abstract

OBJECTIVE

To evaluate the role of human papilloma virus (HPV) infection and inactivation of p16 gene in laryngeal papilloma (LP) and laryngeal squamous cell carcinoma (LC).

METHODS

HPV consensus primers direct in situ polymerase chain reaction (ISPCR) and immunohistochemical method were applied to detect the presence of HPV genomes (1, 6, 8, 11, 13, 16, 18, 30, 31, 32, 33, 45, 51) and the expression of p16 protein respectively in 93 cases of formalin-fixed, paraffin-imbedded specimens, which contained 46 cases of LPs [adult-onset laryngeal papilloma (ALP) 21, juvenile-onset laryngeal papilloma (JLP)25], 26 cases of LCs, 6 cases of normal tissues adjacent to carcinoma, and 15 cases of vocal noduli.

RESULTS

(1) The difference of positive rates of HPV-DNA in JLP group (84%, 21/25) and other groups were statistically significant (chi 2 test, P < 0.05). The difference of positive rates of HPV-DNA in ALPs(38.1%, 8/21), in LCs(19.2%, 5/26), in vocal noduli(0%, 0/15), and in normal tissues adjacent to carcinoma(0%, 0/6) were not significant statistically (chi 2 test or Fisher's exact probability test, P > 0.05). (2) The positive rates of expression of p16 protein in ALP group(57.1%, 12/21) and LC group(38.5%, 10/26) were significantly lower than that in vocal nodule group(93.3%, 14/15), in JLP group(88%, 22/25), and in normal tissues adjacent to carcinoma group (100%, 6/6) (chi 2 test or Fisher's exact probability test, P > 0.05). There were no significant differences of positive rates of expression of p16 protein between ALP group and LC group, and between JLP group and vocal nodule group (chi 2 test, P > 0.05). (3) In LPs, the difference of positive rates of p16 protein expression between HPV positive cases and HPV negative cases was significant statistically (chi 2 test, P < 0.05). In LCs, there was no difference in p16 protein expression rate between the two teams(Fisher exact probability test, P > 0.05).

CONCLUSION

The pathogenesis of JLP is closely associated with HPV infection and not associated with the inactivation of p16 gene. Conversely, the pathogenesis of ALP and LC is associated with the inactivation of p16 gene and not associated with the HPV infection.

摘要

目的

评估人乳头瘤病毒（HPV）感染及p16基因失活在喉乳头状瘤（LP）和喉鳞状细胞癌（LC）中的作用。

方法

采用HPV通用引物直接原位聚合酶链反应（ISPCR）和免疫组化方法，分别检测93例福尔马林固定、石蜡包埋标本中HPV基因组（1、6、8、11、13、16、18、30、31、32、33、45、51）的存在情况及p16蛋白的表达，这些标本包括46例LP[成人型喉乳头状瘤（ALP）21例、幼年型喉乳头状瘤（JLP）25例]、26例LC、6例癌旁正常组织及15例声带小结。

结果

（1）JLP组HPV - DNA阳性率（84%，21/25）与其他组相比差异有统计学意义（χ²检验，P < 0.05）。ALP组（38.1%，8/21）、LC组（19.2%，5/26）、声带小结组（0%，0/15）及癌旁正常组织组（0%，0/6）的HPV - DNA阳性率差异无统计学意义（χ²检验或Fisher确切概率法，P > 0.05）。（2）ALP组（57.1%，12/21）和LC组（38.5%，10/26）p16蛋白表达阳性率显著低于声带小结组（93.3%，14/15）、JLP组（88%，22/25）及癌旁正常组织组（100%，6/6）（χ²检验或Fisher确切概率法，P > 0.05）。ALP组与LC组、JLP组与声带小结组之间p16蛋白表达阳性率差异无统计学意义（χ²检验，P > 0.05）。（3）在LP中，HPV阳性病例与HPV阴性病例p16蛋白表达阳性率差异有统计学意义（χ²检验，P < 0.05）。在LC中，两组间p16蛋白表达率无差异（Fisher确切概率法，P > 0.05）。