König C S, Sutherland I A
Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex UB8 3PH, UK.
J Chromatogr A. 2007 Jun 1;1151(1-2):131-5. doi: 10.1016/j.chroma.2007.02.070. Epub 2007 Feb 24.
In dual-flow counter-current chromatography (DF-CCC), the two immiscible liquids are flowing in opposite directions in the coil. The method allows for the continuous separation of two solutes. In this study a numerical model was developed to allow for the detailed investigation of flow in such columns. The mesh model of the presented DF spiral column was developed in line with an existing experimental model. The paper presents results during the early filling stages for different rotation directions. These clearly illustrate the performance of the developed model by (1) confirming the importance of flowing the lighter phase from tail to head and the heavier phase from head to tail and (2) by visualising mixing waves and the recognised back and forth "swish-swash" motion as present in CCC in that operating mode.
在双流逆流色谱法（DF-CCC）中，两种互不相溶的液体在螺旋管中沿相反方向流动。该方法可实现两种溶质的连续分离。在本研究中，开发了一个数值模型，以便对这类色谱柱中的流动进行详细研究。所呈现的DF螺旋柱的网格模型是根据现有的实验模型建立的。本文给出了不同旋转方向下早期填充阶段的结果。这些结果通过以下方式清楚地说明了所开发模型的性能：（1）证实了轻相从尾部流向头部、重相从头部流向尾部的重要性；（2）通过可视化混合波以及在该操作模式下CCC中存在的公认的来回“涮洗”运动。
