Riachi N J, Harik S I
Department of Neurology, University Hospitals of Cleveland, Ohio.
Exp Neurol. 1992 Feb;115(2):212-7. doi: 10.1016/0014-4886(92)90055-u.
We assessed the two forms of monoamine oxidase (MAO), MAO-A and MAO-B, in discrete regions of the brain and in cerebral micro- and macrovessels, choroid plexus, and liver of three species of monkeys: African Green, rhesus, and cynomolgus. MAO was determined by specific [3H]pargyline binding which is stoichiometric and irreversible and by measuring the rate of oxidation of several substrates. Cerebral micro- and macrovessels had low MAO content. Regional brain MAO did not vary by more than one-fold in the brains of each of the three species of monkeys and was higher in the basal ganglia than in the cerebral cortex or cerebellum. MAO in the choroid plexus was low, while the liver had higher MAO activity than any of the brain samples. The vast majority of MAO in all the tissues that we examined was of the MAO-B type, and specific [3H]pargyline binding correlated well with the oxidation rate of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. These results show marked similarities in brain MAO distribution between monkey and man. Or the three monkey species, the African Green monkey had the lowest MAO activity in its cerebral microvessels, which constitute the blood-brain barrier, although the small number of observations in each group did not allow statistical analyses of the differences.
我们评估了三种猴子（非洲绿猴、恒河猴和食蟹猴）大脑离散区域、脑微血管和大血管、脉络丛以及肝脏中两种形式的单胺氧化酶（MAO），即MAO-A和MAO-B。通过化学计量且不可逆的特异性[3H]帕吉林结合以及测量几种底物的氧化速率来测定MAO。脑微血管和大血管中的MAO含量较低。在这三种猴子的大脑中，区域脑MAO的变化不超过一倍，基底神经节中的MAO含量高于大脑皮层或小脑。脉络丛中的MAO含量较低，而肝脏中的MAO活性高于任何脑样本。在我们检测的所有组织中，绝大多数MAO为MAO-B型，特异性[3H]帕吉林结合与1-甲基-4-苯基-1,2,3,6-四氢吡啶的氧化速率密切相关。这些结果表明猴子和人类大脑中MAO分布存在显著相似性。在这三种猴子中，非洲绿猴构成血脑屏障的脑微血管中的MAO活性最低，不过每组观察数量较少，无法对差异进行统计学分析。
