反复电惊厥发作后大鼠脑中蛋白激酶C的活性及亚细胞分布

Protein kinase C activity and subcellular distribution in rat brain following repeated electroconvulsive seizures.

作者信息

Vernet O, Rostworowski K, Sherwin A L

机构信息

Montreal Neurological Institute, McGill University, Québec, Canada.

出版信息

Exp Neurol. 1992 Feb;115(2):297-301. doi: 10.1016/0014-4886(92)90065-x.

DOI:10.1016/0014-4886(92)90065-x

PMID:1735474

Abstract

Protein kinase C (PKC) activity was measured in samples of neocortex, cerebellum, and hippocampus from adult rats receiving a series of 10 electroconvulsive seizures (ECS). Rats were sacrificed immediately and at various intervals from 15 min to 24 h after the last seizure. From 77 to 84% of total PKC activity was found in the cytosol versus the membrane fraction. PKC activity in cerebellum was significantly higher than in neocortex (15%, P less than 0.05). Repeated ECS treatment did not affect total PKC activity nor its distribution between membrane and cytosolic fractions when compared with sham ECS controls. This finding is in keeping with reports that adrenergic-stimulated phosphoinositol turnover is not altered 24 h following repeated ECS.

摘要

在接受一系列10次电惊厥发作（ECS）的成年大鼠的新皮质、小脑和海马样本中测量蛋白激酶C（PKC）活性。在最后一次发作后的15分钟至24小时内，大鼠被立即处死，并在不同时间间隔处死。与膜部分相比，77%至84%的总PKC活性存在于胞质溶胶中。小脑中的PKC活性显著高于新皮质（15%，P<0.05）。与假ECS对照组相比，重复ECS治疗不影响总PKC活性及其在膜和胞质部分之间的分布。这一发现与重复ECS后24小时肾上腺素能刺激的磷酸肌醇周转率未改变的报道一致。