Prgomet C, Prenner M L, Schwarz F J, Pfaffl M W
Lehrstuhl für Physiologie, Technische Universität München Weihenstephan, Freising, Germany.
J Anim Physiol Anim Nutr (Berl). 2007 Apr;91(3-4):109-19. doi: 10.1111/j.1439-0396.2006.00649.x.
Lactoferrin (LF) is a cationic iron-binding glycoprotein that is abundantly expressed and secreted from glandular epithelial cells and a prominent component of the secondary granules of polymorphonuclear neutrophils. Various in vitro and in vivo experiments demonstrate anti-microbial, -viral, -mycotic and -inflammatory effects of LF, associated with modulations of the immune system. Effects of oral administered LF on selected immune system parameters were studied in calves. Five calves were fed LF beginning on day 3 of life with colostral milk and starting on day 6 of life milk replacer enriched with 0.16% LF was fed. The average daily intake of LF per calf was 1.5-1.6 g/day. Additional five calves served as control group with identical treatment except for the LF supplementation. At the end of the study (day 61 of life), all calves were slaughtered and various tissues were sampled for histological and gene-expression studies. LF given orally was shown to act as an immunomodulatory agent by enhancing the size of Peyer's patches in the ileum and increasing blood serum immunoglobulin G levels. In addition, the number of peripheral blood leucocytes increased and mRNA levels of various interleukins (IL) such as IL-1beta, IL-8, IL-10 and interferon gamma (IFNgamma) in those cells in response to LF treatment were enhanced. In blood, the mRNA expression of the pro-inflammatory marker genes IL-1beta and IFNgamma decreased over 10-week treatment. Additionally, LF feeding decreased villus sizes in the jejunum. Together these findings emphasize the ability of LF to stimulate prominent immune system parameters and that it has the capacity to modulate the immune responses in a positive way.
乳铁蛋白（LF）是一种阳离子铁结合糖蛋白，在腺上皮细胞中大量表达和分泌，是多形核中性粒细胞次级颗粒的重要组成部分。各种体外和体内实验表明，LF具有抗微生物、抗病毒、抗真菌和抗炎作用，与免疫系统的调节有关。研究了口服LF对犊牛选定免疫系统参数的影响。五头犊牛从出生第3天开始用初乳喂食LF，从出生第6天开始喂食添加0.16%LF的代乳品。每头犊牛的LF平均日摄入量为1.5 - 1.6克/天。另外五头犊牛作为对照组，除不补充LF外，处理方式相同。在研究结束时（出生第61天），所有犊牛被屠宰，采集各种组织进行组织学和基因表达研究。结果表明，口服LF可作为一种免疫调节剂，通过增大回肠派尔集合淋巴结的尺寸和提高血清免疫球蛋白G水平发挥作用。此外，外周血白细胞数量增加，LF处理后这些细胞中各种白细胞介素（IL）如IL - 1β、IL - 8、IL - 10和干扰素γ（IFNγ）的mRNA水平升高。在血液中，经过10周的处理，促炎标记基因IL - 1β和IFNγ的mRNA表达下降。此外，喂食LF会使空肠绒毛尺寸减小。这些发现共同强调了LF刺激重要免疫系统参数的能力，以及它以积极方式调节免疫反应的能力。
