Kuhlmann Tanja, Remington Leah, Maruschak Brigitte, Owens Trevor, Brück Wolfgang
Department of Neuropathology, University of Göttingen, Göttingen, Germany.
J Neuropathol Exp Neurol. 2007 Mar;66(3):238-46. doi: 10.1097/01.jnen.0000248559.83573.71.
The unambiguous identification of oligodendrocytes in tissue sections, especially in myelinated tracts, is often difficult. Most of the antibodies used to identify oligodendrocytes label the myelin sheath as well. Originally described as an inhibitor of axonal outgrowth, Nogo-A is known to be strongly expressed in mature oligodendrocytes in vivo. In the present investigation we analyzed the expression patterns of Nogo-A in adult mouse and human CNS as well as in demyelinating animal models and multiple sclerosis lesions. Nogo-A expression was compared with that of other frequently used oligodendroglial markers such as CC1, CNP, and in situ hybridization for proteolipid protein mRNA. Nogo-A strongly and reliably labeled oligodendrocytes in the adult CNS as well as in demyelinating lesions and thus represents a valuable tool for the identification of oligodendrocytes in human and mouse CNS tissue.
在组织切片中，尤其是在有髓神经纤维束中，明确鉴定少突胶质细胞往往很困难。大多数用于鉴定少突胶质细胞的抗体也会标记髓鞘。Nogo-A最初被描述为轴突生长的抑制剂，已知在体内成熟少突胶质细胞中强烈表达。在本研究中，我们分析了Nogo-A在成年小鼠和人类中枢神经系统以及脱髓鞘动物模型和多发性硬化症病变中的表达模式。将Nogo-A的表达与其他常用的少突胶质细胞标记物如CC1、CNP以及蛋白脂蛋白mRNA的原位杂交进行了比较。Nogo-A在成年中枢神经系统以及脱髓鞘病变中强烈且可靠地标记少突胶质细胞，因此是鉴定人类和小鼠中枢神经系统组织中少突胶质细胞的有价值工具。
