Izycka-Swieszewska Ewa, Drozyńska Elzbieta, Rzepko Robert, Dembowska Bozena, Grajkowska Wiesława, Brozyna Agnieszka, Perek Danuta, Balcerska Anna, Jaśkiewicz Kazimierz
Department of Pathomorphology, Medical University of Gdansk, Gdansk, Poland.
Folia Neuropathol. 2007;45(1):1-8.
The aim of the study was to determine microscopic angiogenic parameters of neuroblastoma (NB) Schwannian stroma-poor tumours. Furthermore the associations between vascular parameters and clinicopathological features of tumours and basic prognostic factors were analysed. Examined tissue samples from 62 NB came from 39 untreated and 23 chemotherapy pretreated tumours. The clinicopathological data comprised: patients' age, gender, survival, tumour site and stage, tumour histology and MYCN status.The morphological analysis of the angiogenic potential concentrated on examination of vascular patterns - classical type or pathological angiogenesis with mural microvascular proliferation (MVP). The quantitative study included semi-automatic assessment of vascular density (VD) in CD34 stained tumour sections. Pathologic angiogenesis with MVP, including simple and/or glomeruloid type, was encountered in 25 cases and was more frequent in differentiating histology subtype and extraadrenal tumours. VD value ranged from 56 to 385 vessels/mm2 (median 149). Higher VD was connected with younger patient's age. In untreated tumours VD was significantly higher in infants than in children over one year of age. Pathologic type angiogenesis and lower VD were found to be associated with shorter survival. Our study confirmed high vascularization of NB and revealed common occurrence of vascular pattern with MVP. Angiogenic potential in the analysed group showed diversity related to some clinicopathological tumour features. This points toward heterogeneity of NB tumours in vascular aspects, possibly affecting tumours' reactivity to antiangiogenic therapy.
本研究的目的是确定神经母细胞瘤（NB）中神经鞘基质缺乏型肿瘤的微观血管生成参数。此外，还分析了血管参数与肿瘤临床病理特征及基本预后因素之间的关联。来自62例NB的检测组织样本，其中39例为未经治疗的肿瘤，23例为化疗预处理的肿瘤。临床病理数据包括：患者年龄、性别、生存情况、肿瘤部位和分期、肿瘤组织学及MYCN状态。血管生成潜力的形态学分析集中于血管模式的检查——经典型或伴有壁层微血管增殖（MVP）的病理性血管生成。定量研究包括对CD34染色肿瘤切片中血管密度（VD）的半自动评估。25例出现伴有MVP的病理性血管生成，包括单纯型和/或肾小球样型，在分化型组织学亚型和肾上腺外肿瘤中更常见。VD值范围为56至385个血管/mm²（中位数为149）。较高的VD与患者年龄较小有关。在未经治疗的肿瘤中，婴儿的VD显著高于1岁以上儿童。发现病理性血管生成类型和较低的VD与较短的生存期相关。我们的研究证实了NB的高血管化，并揭示了伴有MVP的血管模式的常见情况。分析组中的血管生成潜力显示出与一些肿瘤临床病理特征相关的多样性。这表明NB肿瘤在血管方面存在异质性，可能影响肿瘤对抗血管生成治疗的反应。
