Stoltenberg M, Larsen A, Doering P, Sadauskas E, Locht L J, Danscher G
Department of Neurobiology, Institute of Anatomy, University of Aarhus, Aarhus C, Denmark.
Histol Histopathol. 2007 Jun;22(6):617-22. doi: 10.14670/HH-22.617.
A short clarifying view of how semiconductor quantum dots (QDs) can be made visible in tissue sections by autometallographic (AMG) silver enhancement and how the introduction of AMG enhanceable gold nanoparticles into isolated cells can be used to follow the fate of these marked cells in organisms and cell cultures. As the AMG approach for visualizing quantum dots is extremely sensitive, QDs less than one nanometer can be made visible at both LM and EM levels.
简要阐述了通过自动金相(AMG)银增强技术使半导体量子点(QDs)在组织切片中可见的方法,以及将可通过AMG增强的金纳米颗粒引入分离细胞后,如何用于追踪这些标记细胞在生物体和细胞培养物中的命运。由于用于可视化量子点的AMG方法极其灵敏,小于一纳米的量子点在光镜和电镜水平下均可被观察到。