Rosner M, Hanneder M, Freilinger A, Hengstschläger M
Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria.
Amino Acids. 2007;32(3):341-5. doi: 10.1007/s00726-007-0509-0. Epub 2007 Mar 16.
The serine/threonine protein kinase Akt (also known as PKB) is a proto-oncogene and one of the most frequently hyperactivated kinases in human cancer. Its activation downstream of growth-factor-stimulated phosphatidylinositide-3'-OH kinase activity plays a role in the control of cell cycle, cell growth, apoptosis and cell energy metabolism. Akt phosphorylates some thousand downstream substrates, including typical cytoplasmic as well as nuclear proteins. Accordingly, it is not surprising that Akt activity can be found in both, the cytoplasm and the nucleus. Here we report the cell cycle regulation of nuclear and cytoplasmic Akt activity in mammalian cells. These data provide new insights into the regulation of Akt activity and have implications for future studies on the regulation of the wide variety of different nuclear and cytoplasmic Akt substrates.
丝氨酸/苏氨酸蛋白激酶Akt（也称为PKB）是一种原癌基因，是人类癌症中最常过度激活的激酶之一。其在生长因子刺激的磷脂酰肌醇-3'-OH激酶活性下游的激活在细胞周期、细胞生长、细胞凋亡和细胞能量代谢的控制中发挥作用。Akt可磷酸化数千种下游底物，包括典型的细胞质蛋白和核蛋白。因此，在细胞质和细胞核中均能发现Akt活性也就不足为奇了。在此，我们报告哺乳动物细胞中核Akt活性和细胞质Akt活性的细胞周期调控。这些数据为Akt活性的调控提供了新的见解，并对未来关于各种不同的核Akt底物和细胞质Akt底物调控的研究具有启示意义。
