Yu Yiqun, Gu Shuting, Huang Hai, Wen Tieqiao
Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, 99 Shang Da Road, Shanghai 200444, China.
J Neurol Sci. 2007 Apr 15;255(1-2):81-6. doi: 10.1016/j.jns.2007.01.076. Epub 2007 Mar 13.
Neural stem cells (NSCs) are self-renewing and pluripotent, which can differentiate into neurons, astrocytes and oligodendrocytes. Due to these properties, NSCs are supposed to be an ideal candidate to clinical purpose while research on cell replacement therapy to treat neural diseases has been widely investigated recently. In this article, we demonstrated a new and efficient method to induce the generation of proliferative dopaminergic neurons from rat NSCs in the presence of bFGF, heparin and laminin both in vitro and in vivo. These cells were testified to survive in the grafted 6-Hydroxy-Dopamine (6-OHDA) lesioned rat for at least 1 month. More importantly, migration to close host tissue was observed on day 30 post-transplantation. In this regard, we anticipated that this technology may advance stem cell-based therapy to replace lost neurons in neural injury or neurodegenerative disorders.
神经干细胞（NSCs）具有自我更新和多能性，能够分化为神经元、星形胶质细胞和少突胶质细胞。基于这些特性，神经干细胞被认为是临床应用的理想候选者，近年来关于细胞替代疗法治疗神经疾病的研究也得到了广泛开展。在本文中，我们展示了一种新的高效方法，即在体外和体内在碱性成纤维细胞生长因子（bFGF）、肝素和层粘连蛋白存在的情况下，诱导大鼠神经干细胞产生增殖性多巴胺能神经元。这些细胞在移植到6-羟基多巴胺（6-OHDA）损伤大鼠体内后至少存活了1个月。更重要的是，在移植后第30天观察到它们迁移至邻近的宿主组织。在这方面，我们预计这项技术可能会推动基于干细胞的疗法，以替代神经损伤或神经退行性疾病中丢失的神经元。
