Wu Ping, Tarasenko Yevgeniya I, Gu Yanping, Huang Li-Yen M, Coggeshall Richard E, Yu Yongjia
Department of Anatomy, University of Texas Medical Branch, Galveston, Texas 77555, USA.
Nat Neurosci. 2002 Dec;5(12):1271-8. doi: 10.1038/nn974.
Pluripotent or multipotent stem cells isolated from human embryos or adult central nervous system (CNS) may provide new neurons to ameliorate neural disorders. A major obstacle, however, is that the majority of such cells do not differentiate into neurons when grafted into non-neurogenic areas of the adult CNS. Here we report a new in vitro priming procedure that generates a nearly pure population of neurons from fetal human neural stem cells (hNSCs) transplanted into adult rat CNS. Furthermore, the grafted cells differentiated by acquiring a cholinergic phenotype in a region-specific manner. This technology may advance stem cell-based therapy to replace lost neurons in neural injury or neurodegenerative disorders.
从人类胚胎或成体中枢神经系统（CNS）分离出的多能或多潜能干细胞，可能会提供新的神经元以改善神经疾病。然而，一个主要障碍是，当将大多数此类细胞移植到成体CNS的非神经源性区域时，它们不会分化为神经元。在此，我们报告一种新的体外预处理程序，该程序可从移植到成年大鼠CNS中的胎儿人类神经干细胞（hNSCs）产生几乎纯的神经元群体。此外，移植的细胞通过以区域特异性方式获得胆碱能表型而发生分化。这项技术可能会推动基于干细胞的疗法，以替代神经损伤或神经退行性疾病中丢失的神经元。
