Bengtsson Simon, Werker Alan, Christensson Magnus, Welander Thomas
AnoxKaldnes AB, Klosterängsvägen 11A, SE-226 47 Lund, Sweden.
Bioresour Technol. 2008 Feb;99(3):509-16. doi: 10.1016/j.biortech.2007.01.020. Epub 2007 Mar 13.
Production of polyhydroxyalkanoates (PHAs) in activated sludge treating wastewater represents an economical and environmental promising alternative to pure culture fermentations. A process for production of PHA from a paper mill wastewater was examined at laboratory scale. The three stage process examined consisted of acidogenic fermentation to convert wastewater organic matter to volatile fatty acids (VFAs), an activated sludge system operating under feast/famine conditions to enrich for PHA producing organisms and accumulation of PHA in batch experiments. After fermentation of the wastewater, 74% of the soluble COD was present as VFA (acetate, propionate, butyrate and valerate) and the resulting PHA after batch accumulation consisted of 31-47 mol% hydroxybutyrate and 53-69 mol% hydroxyvalerate. The maximum PHA content achieved was 48% of the sludge dry weight and the three stage process exhibited a potential to produce 0.11 kg of PHA per kg of influent COD treated.
在处理废水的活性污泥中生产聚羟基脂肪酸酯（PHA）是一种经济且具有环境前景的纯培养发酵替代方法。在实验室规模下研究了一种从造纸厂废水中生产PHA的工艺。所研究的三阶段工艺包括：产酸发酵，将废水有机物转化为挥发性脂肪酸（VFA）；活性污泥系统在 feast/famine 条件下运行，以富集PHA产生菌；以及在分批实验中积累PHA。废水发酵后，74%的可溶性化学需氧量（COD）以VFA（乙酸盐、丙酸盐、丁酸盐和戊酸盐）形式存在，分批积累后得到的PHA由31 - 47摩尔%的羟基丁酸酯和53 - 69摩尔%的羟基戊酸酯组成。实现的最大PHA含量为污泥干重的48%，该三阶段工艺显示出每处理1千克进水COD有生产0.11千克PHA的潜力。
