Wani Revati, Kodam K M, Gawai K R, Dhakephalkar P K
Biochemistry Division, Department of Chemistry, University of Pune, Ganeshkhind, Pune, 411007, India.
Appl Microbiol Biotechnol. 2007 Jun;75(3):627-32. doi: 10.1007/s00253-007-0862-7. Epub 2007 Mar 15.
The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.
从洛纳尔碱性火山口湖分离出的 Cr(VI) 还原细菌菌株 MCMB-821 被鉴定为洋葱伯克霍尔德菌。MCMB-821 对 1000 ppm 的 Cr(VI) 具有抗性，在 pH 9.0、2% 盐存在且以乳糖作为电子供体的条件下，36 小时内可还原 75 ppm Cr(VI) 的 98%。MCMB-821 的铬酸盐还原效率在好氧和厌氧条件下相当。电子顺磁共振光谱数据表明，MCMB-821 通过形成瞬态 Cr(V) 中间体将 Cr(VI) 还原为 Cr(III)。MCMB-821 的铬酸盐还原能力在膜抑制剂存在下受到抑制，而在 2,4-二硝基苯酚存在下增强，这表明电子传递链参与了 Cr(VI) 的生物还原过程。
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