[利用荧光光谱法研究环丙沙星探针与小牛胸腺DNA的相互作用]

[Study of interaction of ciprofloxacin probe and calf thymus DNA using fluorescence spectrometry].

作者信息

Gan Ning, Ge Cong-xin

机构信息

College of Material Science and Chemical Engineering, Department of Chemistry, Ningbo University, Ningbo, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Dec;26(12):2276-9.

PMID:17361729

Abstract

The interaction of ciprofloxacin and calf thymus DNA (ctDNA) was studied using UV spectrometry and fluorescence spectrometry. Ciprofloxacin exhibited a fluorescence excitation at 270 nm and a fluorescence emission at 420 nm in pH 7.0 phosphate buffer solutions due to the groove binding of ciprofloxacin on calf thymus ctDNA. The binding constant was 2. 64 X 104 mol(-1) x L(25 degrees C). Based on the fluorescence quenching, a novel method for sensitive determination of calf thymus ctDNA concentration ranging from 80 nmol x L(-1) to 45 micromol x L(-1) was developed. The relative standard deviation for eleven detections of 25 micromol x L(-1) ctDNA was 4. 2%.

摘要

采用紫外光谱法和荧光光谱法研究了环丙沙星与小牛胸腺DNA（ctDNA）的相互作用。在pH 7.0的磷酸盐缓冲溶液中，由于环丙沙星与小牛胸腺ctDNA的沟槽结合，环丙沙星在270 nm处表现出荧光激发，在420 nm处表现出荧光发射。结合常数为2.64×10⁴ mol⁻¹×L（25℃）。基于荧光猝灭，建立了一种灵敏测定小牛胸腺ctDNA浓度范围为80 nmol×L⁻¹至45 μmol×L⁻¹的新方法。对25 μmol×L⁻¹ ctDNA进行11次检测的相对标准偏差为4.2%。