Qiu Bin, Guo Longhua, Chen Zhitao, Chi Yuwu, Zhang Lan, Chen Guonan
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fuzhou 350002, China.
Biosens Bioelectron. 2009 Jan 1;24(5):1281-5. doi: 10.1016/j.bios.2008.07.055. Epub 2008 Aug 5.
A novel N-4-butylamine acridone (BAA), which is an acridone derivative was synthesized and characterized by IR, MS and (1)H NMR. The fluorescent characteristics of BAA was investigated in detail and used as the fluorescent probe for detection of calf thymus DNA (ctDNA). It was found that DNA was able to quench the fluorescence of BAA at 426 nm with the excitation at 254 nm. Under optimal conditions, the corresponding linear response range was from 1.0 to 20.0mg/L and the limit of detections (LOD) was 0.020 mg/L (defined as S/N=3). Moreover, the interaction between BAA and ctDNA was investigated by fluorescence, absorption and viscosity measurements. The results suggested that the interaction between BAA and ctDNA is groove binding in nature.
合成了一种新型的N-4-丁胺吖啶酮（BAA），它是一种吖啶酮衍生物，并通过红外光谱（IR）、质谱（MS）和核磁共振氢谱（¹H NMR）对其进行了表征。详细研究了BAA的荧光特性，并将其用作检测小牛胸腺DNA（ctDNA）的荧光探针。发现在254nm激发下，DNA能够猝灭BAA在426nm处的荧光。在最佳条件下，相应的线性响应范围为1.0至20.0mg/L，检测限（LOD）为0.020mg/L（定义为S/N = 3）。此外，通过荧光、吸收和粘度测量研究了BAA与ctDNA之间的相互作用。结果表明，BAA与ctDNA之间的相互作用本质上是沟槽结合。
