Humiczewska M
Folia Histochem Cytochem (Krakow). 1975;13(3-4):213-29.
The object of the study was the investigation of the occurrence and localization of oxidative enzymes in the 4th and 5th larval stages of the liver fluke, i. e. in the cercaria and metacercaria. The following enzymes were detected: cytochrome oxidase, peroxidase, NADH and NADPH tetrazolium reductases/diaphorases) as well as succinate, isocitrate, malate, lactate, alpha-glycerophosphate, glyceraldehyde phosphate, glucose-6-phosphate, 6-phosphogluconate, beta-hydroxybutyrate, L-glutamate and alcohol dehydrogenases. The occurrence and localization of the enzymes were investigated histochemically in the cercaria still unreleased from the snail tissues, in the free natatorial cercaria, and in the encysted specimen, i.e. metacercaria. Among the enzymes studied only peroxidase was found to be absent from the cercaria and metacercaria, the latter larva being deprived of alcohol and L-glutamate dehydrogenases as well. The aerobic path, i.e. the Krebs cycle, was ascertained as the principal mode of obtaining energy in the free natatorial cercaria and metacercaria, and glycolysis as the main energy path for the undetached larva. The analysis of the above-mentioned oxidative enzymes was the basis for the description, within the available range, of the function and metabolism of individual organs of cercaria in different periods of its development. In the recapitulation the author discusses the effect of the parasitic way of life of the larval forms of Fasciola hepatica on their energy metabolism.
本研究的目的是调查肝片吸虫第四和第五幼虫阶段，即尾蚴和后尾蚴中氧化酶的发生情况和定位。检测到以下几种酶：细胞色素氧化酶、过氧化物酶、NADH和NADPH四氮唑还原酶/黄递酶）以及琥珀酸、异柠檬酸、苹果酸、乳酸、α-甘油磷酸、甘油醛磷酸、6-磷酸葡萄糖、6-磷酸葡萄糖酸、β-羟基丁酸、L-谷氨酸和乙醇脱氢酶。采用组织化学方法研究了仍未从蜗牛组织中释放出来的尾蚴、自由游动的尾蚴以及包囊标本即后尾蚴中这些酶的发生情况和定位。在所研究的酶中，仅发现尾蚴和后尾蚴中缺乏过氧化物酶，后一种幼虫还缺乏乙醇和L-谷氨酸脱氢酶。需氧途径，即三羧酸循环，被确定为自由游动的尾蚴和后尾蚴获取能量的主要方式，而糖酵解是未脱离的幼虫的主要能量途径。对上述氧化酶的分析是在现有范围内描述尾蚴不同发育时期各个器官功能和代谢的基础。在总结部分，作者讨论了肝片吸虫幼虫寄生生活方式对其能量代谢的影响。
