纳米内径毛细管电泳分析哺乳动物细胞质
Analysis of Mammalian Cell Cytoplasm with Electrophoresis in Nanometer Inner Diameter Capillaries.
作者信息
Woods Lori A, Powell Paula R, Paxon Tracy L, Ewing Andrew G
机构信息
Department of Chemistry, 104 Chemistry Building, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
出版信息
Electroanalysis. 2005 Jul;17(13):1192-1197. doi: 10.1002/elan.200403240.
Abstract
Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240 ± 60 μM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.
摘要
内径770纳米的毛细管电泳与带有与蚀刻毛细管相匹配的蚀刻电极的电化学检测相结合（蚀刻电化学检测），已用于超微量进样，以从完整的单个哺乳动物细胞中注入亚细胞样品。已实现从大鼠嗜铬细胞瘤细胞中获取的细胞质样品的分离。单个细胞总体积中低至8%的样品已被采样和分析。使用该技术已在这些PC12细胞中鉴定并定量了多巴胺。已确定大鼠嗜铬细胞瘤细胞中多巴胺的平均细胞质水平为240±60μM。使用内径770纳米的毛细管电泳结合电化学检测在单细胞水平监测细胞质神经递质，可提供有关诸如神经递质储存和合成等复杂细胞功能的信息。
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